Rabbit anti-Human Mps1/TTK Polyclonal Antibody | anti-TTK antibody

Anti-Mps1/TTK Antibody, Rabbit Polyclonal

Cat. Num. AAA8100882

• Gene Names: RPS27; S27; MPS1; MPS-1
• Reactivity: Human
• Applications: Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: Mps1/TTK; Polyclonal Antibody; Anti-Mps1/TTK Antibody; Rabbit Polyclonal; TTK Antibody; Rabbit PAb; Antigen Affinity Purified; TTK protein kinase; Anti-CT96 Antibody; Anti-ESK Antibody; Anti-MPH1 Antibody; Anti-MPS1 Antibody; Anti-MPS1L1 Antibody; Anti-PYT Antibody; anti-TTK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human TTK
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-TTK antibody: Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: IHC-P: 1:500-1:2000; ICC/IF: 1:300-1:10,000
• Immunogen: A synthetic peptide corresponding to the N-terminus of the Human TTK
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human TTK, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human RPS27 in human lung cancer with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human RPS27 in human brain with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

Immunofluorescence (IF)

(Immunofluorescence staining of TTK in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human TTK polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)

Related Product Information for anti-TTK antibody

TTK protein kinase (TTK), also known as Monopolar spindle 1 (MPS1), is a key regulator of the spindle assembly checkpoint (SAC), which functions to maintain genomic integrity. TTK has emerged as a promising therapeutic target in human cancers, including triple-negative breast cancer (TNBC). TTK (Mps1) is a key regulator of chromosome segregation and is the subject of novel targeted therapy approaches by small-molecule inhibitors. The dual-specificity protein kinase TTK (TTK) is the most up-regulated and differentially expressed kinase encoding genes in GSCs. Functionally, TTK was essential for in vitro clonogenicity and in vivo tumor propagation in GSCs. Clinically, TTK expression was highly enriched in GBM, moreover, was inversely correlated with a poor prognosis in GBM patients. Mechanistically, mitochondrial fission regulator 2 (MTFR2) was identified as one of the most correlated genes to TTK and transcriptionally regulated TTK expression via activation of TTK promoter. Collectively, MTFR2-dependent regulation of TTK plays a key role in maintaining GSCs in GBM and is a potential novel druggable target for glioblastoma (GBM).

NCBI and Uniprot Product Information

• NCBI GI #: 4506711
• NCBI GeneID: 6232
• NCBI Accession #: NP_001021.1
• NCBI GenBank Nucleotide #: NM_001030.4
• UniProt Accession #: P42677; Q5T4L6
• Molecular Weight: 9,461 Da
• NCBI Official Full Name: 40S ribosomal protein S27
• NCBI Official Synonym Full Names: ribosomal protein S27
• NCBI Official Symbol: RPS27
• NCBI Official Synonym Symbols: S27; MPS1; MPS-1
• NCBI Protein Information: 40S ribosomal protein S27
• UniProt Protein Name: 40S ribosomal protein S27
• Protein Family: Protein tramtrack
• UniProt Gene Name: RPS27
• UniProt Synonym Gene Names: MPS1; MPS-1

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S27E family of ribosomal proteins. It contains a C4-type zinc finger domain that can bind to zinc. The encoded protein has been shown to be able to bind to nucleic acid. It is located in the cytoplasm as a ribosomal component, but it has also been detected in the nucleus. Studies in rat indicate that ribosomal protein S27 is located near ribosomal protein S18 in the 40S subunit and is covalently linked to translation initiation factor eIF3. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

RPS27: a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S27E family of ribosomal proteins. It contains a C4-type zinc finger domain that can bind to zinc. The encoded protein has been shown to be able to bind to nucleic acid. It is located in the cytoplasm as a ribosomal component, but it has also been detected in the nucleus. Studies in rat indicate that ribosomal protein S27 is located near ribosomal protein S18 in the 40S subunit and is covalently linked to translation initiation factor eIF3. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Protein type: Ribosomal; Translation

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: cytosol; nucleoplasm; nucleus

Molecular Function: protein binding; structural constituent of ribosome

Biological Process: mRNA catabolic process, nonsense-mediated decay; ribosomal small subunit assembly and maintenance; rRNA processing; sister chromatid cohesion; SRP-dependent cotranslational protein targeting to membrane; translation; translational initiation; viral transcription