Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Sodium- and chloride-dependent glycine transporter 1 (SLC6A9) Standard Curve.)
Human Sodium-And Chloride-Dependent Glycine Transporter 1 (SLC6A9) ELISA Kit | SLC6A9 elisa kit
Human Sodium-And Chloride-Dependent Glycine Transporter 1 (SLC6A9) ELISA Kit
Gene Names
SLC6A9; GLYT1; GCENSG
Reactivity
Human
Synonyms
Sodium-And Chloride-Dependent Glycine Transporter 1 (SLC6A9); Human Sodium-And Chloride-Dependent Glycine Transporter 1 (SLC6A9) ELISA Kit; SLC6A9; DKFZp547A1118; GLYT1; sodium-and chloride-dependent glycine transporter 1; solute carrier family 6 member 9; SLC6A9 elisa kit
Reactivity
Human
Specificity
Human Sodium- and chloride-dependent glycine transporter 1 (SLC6A9) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC6A9. No significant cross-reactivity or interference between Human SLC6A9 and analogues was observed.
Sequence Length
633
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Sodium- and chloride-dependent glycine transporter 1 (SLC6A9) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Sodium- and chloride-dependent glycine transporter 1 (SLC6A9) Standard Curve.)
Related Product Information for SLC6A9 elisa kit
Description: This Human Sodium- and chloride-dependent glycine transporter 1 (SLC6A9) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC6A9 in samples. An antibody specific for SLC6A9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SLC6A9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC6A9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC6A9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Glycine transport is mediated by 2 sodium-dependent carriers, GLYT1 and GLYT2, that have distinct tissue distributions. While the 3-prime sequences of these 2 cDNAs were identical, the 5-prime noncoding regions and the N termini were completely different. GLYT1b was found only in the white matter of the CNS, while GLYT1a was found in the gray matter of the CNS as well as in macrophages and mast cells in peripheral tissues. Tissue-specific alternative splicing or alternative promoter usage from a single gene resulted in 2 mRNA products encoding similar but distinct glycine transporters. The anatomic distribution of GLYT1a mRNA supported the emerging status of glycine as a supraspinal neurotransmitter and suggested that glycine may function as a chemical messenger outside the CNS
Background: Glycine transport is mediated by 2 sodium-dependent carriers, GLYT1 and GLYT2, that have distinct tissue distributions. While the 3-prime sequences of these 2 cDNAs were identical, the 5-prime noncoding regions and the N termini were completely different. GLYT1b was found only in the white matter of the CNS, while GLYT1a was found in the gray matter of the CNS as well as in macrophages and mast cells in peripheral tissues. Tissue-specific alternative splicing or alternative promoter usage from a single gene resulted in 2 mRNA products encoding similar but distinct glycine transporters. The anatomic distribution of GLYT1a mRNA supported the emerging status of glycine as a supraspinal neurotransmitter and suggested that glycine may function as a chemical messenger outside the CNS
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
sodium- and chloride-dependent glycine transporter 1 isoform 3
NCBI Official Synonym Full Names
solute carrier family 6 member 9
NCBI Official Symbol
SLC6A9
NCBI Official Synonym Symbols
GLYT1; GCENSG
NCBI Protein Information
sodium- and chloride-dependent glycine transporter 1
UniProt Protein Name
Sodium- and chloride-dependent glycine transporter 1
Protein Family
UniProt Gene Name
SLC6A9
UniProt Synonym Gene Names
GlyT-1; GlyT1
NCBI Description
The amino acid glycine acts as an inhibitory neurotransmitter in the central nervous system. The protein encoded by this gene is one of two transporters that stop glycine signaling by removing it from the synaptic cleft. [provided by RefSeq, Jun 2016]
Uniprot Description
Terminates the action of glycine by its high affinity sodium-dependent reuptake into presynaptic terminals. May play a role in regulation of glycine levels in NMDA receptor-mediated neurotransmission.
Research Articles on SLC6A9
Similar Products
Product Notes
The Human SLC6A9 slc6a9 (Catalog #AAA9714255) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714255 ELISA Kit recognizes Human SLC6A9. It is sometimes possible for the material contained within the vial of "Sodium-And Chloride-Dependent Glycine Transporter 1 (SLC6A9), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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