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Western Blot (WB) (Western Blot: Sample: Recombinant MCP2, Mouse.)

Rabbit Monocyte Chemotactic Protein 2 (MCP2) Polyclonal Antibody | anti-MCP2 antibody

Polyclonal Antibody to Monocyte Chemotactic Protein 2 (MCP2)

Gene Names
Ccl8; HC14; Mcp2; MCP-2; Scya8; AB023418; 1810063B20Rik
Reactivity
Mus musculus (Mouse)
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Monocyte Chemotactic Protein 2 (MCP2); Polyclonal Antibody; Polyclonal Antibody to Monocyte Chemotactic Protein 2 (MCP2); anti-MCP2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mus musculus (Mouse)
Clonality
Polyclonal
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
0.45mg/ml (varies by lot)
Applicable Applications for anti-MCP2 antibody
Western Blot (WB), Immunohistochemistry (IHC) - Formalin/Paraffin, Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
Western blotting: 0.5-2ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Source
Polyclonal antibody preparation
Traits
Liquid
Immunogen
Recombinant MCP2 (Glu20~Ile94 (Accession # Q9Z121)) expressed in E.coli (MBS2018474)
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4°C for frequent use.
Aliquot and store at -20°C.

Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Recombinant MCP2, Mouse.)

Western Blot (WB) (Western Blot: Sample: Recombinant MCP2, Mouse.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P;Samples: Mouse Testis Tissue.)

Immunohistochemistry (IHC) (DAB staining on IHC-P;Samples: Mouse Testis Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P;Samples: Mouse Brain Tissue;Primary Ab: 10ug/ml Rabbit Anti-Mouse MCP2 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047 ))

Immunohistochemistry (IHC) (DAB staining on IHC-P;Samples: Mouse Brain Tissue;Primary Ab: 10ug/ml Rabbit Anti-Mouse MCP2 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047 ))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
C-C motif chemokine 8
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 8
NCBI Official Symbol
Ccl8
NCBI Official Synonym Symbols
HC14; Mcp2; MCP-2; Scya8; AB023418; 1810063B20Rik
NCBI Protein Information
C-C motif chemokine 8
UniProt Protein Name
C-C motif chemokine 8
UniProt Gene Name
Ccl8
UniProt Synonym Gene Names
Mcp2; Scya8; MCP-2

Uniprot Description

CCL8: Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Chemokine; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11 C|11 49.91 cM

Cellular Component: extracellular region; extracellular space

Molecular Function: CCR chemokine receptor binding; chemokine activity; cytokine activity; heparin binding

Biological Process: chemotaxis; G-protein coupled receptor signaling pathway; immune response; inflammatory response; lymphocyte chemotaxis; monocyte chemotaxis; neutrophil chemotaxis; positive regulation of GTPase activity; positive regulation of myoblast differentiation

Research Articles on MCP2

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Product Notes

The MCP2 ccl8 (Catalog #AAA2016564) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Monocyte Chemotactic Protein 2 (MCP2) reacts with Mus musculus (Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's Monocyte Chemotactic Protein 2 (MCP2) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) - Formalin/Paraffin, Immunocytochemistry (ICC), Immunoprecipitation (IP) . Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the MCP2 ccl8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Monocyte Chemotactic Protein 2 (MCP2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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