Chicken GAP43 Polyclonal Antibody | anti-GAP43 antibody

Anti-GAP-43

Cat. Num. AAA502179

• Gene Names: Gap43; Basp2
• Reactivity: Bovine , Human, Mouse, Pig, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Total IgY fraction
• Synonyms: GAP43; Polyclonal Antibody; Anti-GAP-43; anti-GAP43 antibody

• Host: Chicken
• Reactivity: Bovine , Human, Mouse, Pig, Rat
• Clonality: Polyclonal
• Specificity: Specific for the ~43k GAP-43 protein.
• Purity/Purification: Total IgY fraction
• Form/Format: Total IgY fraction in PBS + 10 mM NaN3.
• Sequence Length: 226

• Applicable Applications for anti-GAP43 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:2000; IF: 1:500
• Packaging: Adequate amount of material to conduct 10-mino Western Blots.
• Antigen: Synthetic peptide corresponding to amino acid residues from the C-terminal region of the rat GAP43 sequence.
• Antigen Specificity: Specific for endogenous levels of the !43 kDa GAP-43 protein
• Immunogen Information: Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH
• Immunogen Species: Rat
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with human, mouse and rat tissue.
• Biological Significance: GAP-43 is thought to have an important role in development and plasticity because it is expressed at high levels in neuronal growth cones during development and during axonal regeneration (Benowitz and Routtenberg, 1997). There is also evidence from knockout animals that GAP-43 serves to amplify pathfinding signals from the growth cone (Strittmatter et al., 1995). GAP-43 is thought to mediate at least some of these effects via interaction with actin. Importantly, phosphorylation at Ser41 by protein kinase C modulates the interaction of GAP-43 with actin (He et al., 1997) and may also affect neurotransmitter release during forms of plasticity like LTP (Hulo et al., 2002).
• Preparation and Storage: For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year

Testing Data

Related Product Information for anti-GAP43 antibody

Chicken polyclonal antibody

References

• Benowitz LI, Routtenberg A (1997) Gap-43: An intrinsic determinant of neuronal development and plasticit. Trends Neurosci 20:84-91.
• He, Q, Dent, EW, Meiri, KF (1997) Modulation of actin filament behavior by Gap-43 (neuromodulin) is dependent on the phosphorylation status of serine 41, the protein kinase C site. J Neurosci 17:3515-3524.
• Hulo S, Alberi, S, Laux T, Muller D, Caroni P (2002) A point mutant of Gap-43 induces enhanced short-term and long-term hippocampal plasticity. Eur J Neurosci 15:1976-1982.
• Strittmatter SM, Fankhauser C, Huang PL, Mashimo H, Fishman MC (1995) Neuronal path finding is abnormal in mice lacking the neuronal growth cone protein Gap-43," Cell 80:445-452.
• Rayudu Gopalakrishna, Usha Gundimeda, Jason Eric Schiffman, and Thomas H. McNeill (2008) A Direct Redox Regulation of Protein Kinase C Isoenzymes Mediates Oxidant-induced Neuritogenesis in PC12 Cells J. Biol. Chem., May 2008; 283: 14430 - 14444.

NCBI and Uniprot Product Information

• NCBI GI #: 8393415
• NCBI GeneID: 29423
• NCBI Accession #: NP_058891.1
• NCBI GenBank Nucleotide #: NM_017195.3
• UniProt Accession #: P07936; A0JPM4
• Molecular Weight: ~43
• NCBI Official Full Name: neuromodulin
• NCBI Official Synonym Full Names: growth associated protein 43
• NCBI Official Symbol: Gap43
• NCBI Official Synonym Symbols: Basp2
• NCBI Protein Information: neuromodulin; protein F1; growth-associated protein 43; axonal membrane protein GAP-43; growth accentuating protein 43; brain abundant, membrane attached signal protein 2
• UniProt Protein Name: Neuromodulin
• Protein Family: Neuromodulin
• UniProt Gene Name: Gap43
• UniProt Entry Name: NEUM_RAT

NCBI Description

may play a role in neurite outgrowth and nerve regeneration [RGD, Feb 2006]

Uniprot Description

Function: This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction

By similarity.

Subunit structure: Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN

By similarity. Binds calmodulin with a greater affinity in the absence of Ca2+ than in its presence

By similarity.

Subcellular location: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection › growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction › synapse. Cell projection › filopodium membrane; Peripheral membrane protein

By similarity. Note: Cytoplasmic surface of growth cone and synaptic plasma membranes.

Post-translational modification: Palmitoylation by ARF6 is essential for plasma membrane association and axonal and dendritic filopodia induction. Deacylated by LYPLA2

By similarity.Phosphorylated at Ser-41 by PHK. Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity. Ref.7 Ref.8 Ref.9

Sequence similarities: Belongs to the neuromodulin family.Contains 1 IQ domain.