Western Blot (WB)
(Western Blot analysis of 293T, COLO205 cells using p53 (Mono-Methyl-Lys370) Polyclonal Antibody at dilution of 1:1000.)
Rabbit anti-Human Mono-Methyl-p53 Polyclonal Antibody | anti-p53 antibody
Mono-Methyl-p53 (Lys370) Polyclonal Antibody
ELISA: 1:10000
Western Blot (WB)
(Western Blot analysis of 293T, COLO205 cells using p53 (Mono-Methyl-Lys370) Polyclonal Antibody at dilution of 1:1000.)
Western Blot (WB)
(Western Blot analysis of 293T, COLO205 cells using p53 (Mono-Methyl-Lys370) Polyclonal Antibody at dilution of 1:1000.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277). [provided by RefSeq, Dec 2016]
Uniprot Description
p53: a transcription factor and major tumor suppressor that plays a major role in regulating cellular responses to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis. More than 50 percent of human tumors contain a mutation or deletion of the TP53 gene. p53 is modified post-translationally at multiple sites. DNA damage induces phosphorylation of p53 at S15, S20 and S37, reducing its interaction with the oncoprotein MDM2. MDM2 inhibits p53 accumulation by targeting it for ubiquitination and proteasomal degradation. Phosphorylated by many kinases including Chk2 and Chk1 at S20, enhancing its tetramerization, stability and activity. The phosphorylation by CAK at S392 is increased in human tumors and has been reported to influence the growth suppressor function, DNA binding and transcriptional activation of p53. Phosphorylation of p53 at S46 regulates the ability of p53 to induce apoptosis. The acetylation of p53 appears to play a positive role in the accumulation of p53 during the stress response. Following DNA damage, p53 becomes acetylated at K382, enhancing its binding to DNA. Deacetylation of p53 can occur through interaction with SIRT1, a deacetylase that may be involved in cellular aging and the DNA damage response. p53 regulates the transcription of a set of genes encoding endosomal proteins that regulate endosomal functions. These include STEAP3 and CHMP4C, which enhance exosome production, and CAV1 and CHMP4C, which produce a more rapid endosomal clearance of the EGFR from the plasma membrane. DNA damage regulates a p53-mediated secretory pathway, increasing the secretion of some proteins such as Hsp90, SERPINE1, SERPINB5, NKEF-A, and CyPA, and inhibiting the secretion of others including CTSL and IGFBP-2. Two alternatively spliced human isoforms have been reported. Isoform 2 is expressed in quiescent lymphocytes. Seems to be non-functional. May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Protein type: Activator; DNA-binding; Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Transcription factor; Tumor suppressor
Chromosomal Location of Human Ortholog: 17p13.1
Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; intracellular; mitochondrial matrix; mitochondrion; nuclear body; nuclear chromatin; nuclear matrix; nucleolus; nucleoplasm; nucleus; PML body; protein complex; replication fork; transcription factor TFIID complex
Molecular Function: ATP binding; chaperone binding; chromatin binding; copper ion binding; damaged DNA binding; DNA binding; DNA binding transcription factor activity; enzyme binding; histone acetyltransferase binding; histone deacetylase binding; identical protein binding; mRNA 3'-UTR binding; p53 binding; protease binding; protein binding; protein C-terminus binding; protein heterodimerization activity; protein kinase binding; protein N-terminus binding; protein phosphatase 2A binding; protein phosphatase binding; protein self-association; receptor tyrosine kinase binding; transcription factor binding; transcription regulatory region DNA binding; ubiquitin protein ligase binding; zinc ion binding
Biological Process: autophagy; base-excision repair; cell aging; cell cycle arrest; cell differentiation; cell proliferation; cellular response to DNA damage stimulus; cellular response to gamma radiation; cellular response to glucose starvation; cellular response to heat; cellular response to hypoxia; cellular response to ionizing radiation; cellular response to reactive oxygen species; cellular response to UV; chromatin assembly; circadian behavior; determination of adult lifespan; DNA damage response, signal transduction by p53 class mediator; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; DNA strand renaturation; entrainment of circadian clock by photoperiod; ER overload response; G1 DNA damage checkpoint; intrinsic apoptotic signaling pathway by p53 class mediator; intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator; mRNA transcription; multicellular organism development; negative regulation of apoptosis; negative regulation of cell growth; negative regulation of cell proliferation; negative regulation of fibroblast proliferation; negative regulation of helicase activity; negative regulation of smooth muscle cell proliferation; negative regulation of telomerase activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; nucleotide-excision repair; oxidative stress-induced premature senescence; positive regulation of apoptosis; positive regulation of cell cycle arrest; positive regulation of execution phase of apoptosis; positive regulation of gene expression; positive regulation of histone deacetylation; positive regulation of intrinsic apoptotic signaling pathway; positive regulation of leukocyte migration; positive regulation of neuron apoptotic process; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein export from nucleus; positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway; positive regulation of protein oligomerization; positive regulation of reactive oxygen species metabolic process; positive regulation of release of cytochrome c from mitochondria; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress; positive regulation of transcription, DNA-templated; proteasomal ubiquitin-dependent protein catabolic process; protein complex assembly; protein deubiquitination; protein homotetramerization; protein localization; protein tetramerization; Ras protein signal transduction; regulation of apoptosis; regulation of cell cycle G2/M phase transition; regulation of intracellular pH; regulation of mitochondrial membrane permeability; regulation of transcription, DNA-templated; replicative senescence; response to amino acid; response to antibiotic; response to caffeine; response to cytokine; response to ethanol; response to gamma radiation; response to hyperoxia; response to metal ion; response to retinoic acid; response to UV-B; response to vitamin B3; response to X-ray; signal transduction by p53 class mediator; transcription from RNA polymerase II promoter; viral process; wound healing
Disease: Adrenocortical Carcinoma, Hereditary; Basal Cell Carcinoma, Susceptibility To, 7; Breast Cancer; Colorectal Cancer; Glioma Susceptibility 1; Hepatocellular Carcinoma; Li-fraumeni Syndrome 1; Nasopharyngeal Carcinoma; Osteogenic Sarcoma; Pancreatic Cancer; Papilloma Of Choroid Plexus
Research Articles on p53
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Product Notes
The p53 tp53 (Catalog #AAA2549846) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Mono-Methyl-p53 (Lys370) Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Mono-Methyl-p53 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:2000 ELISA: 1:10000. Researchers should empirically determine the suitability of the p53 tp53 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mono-Methyl-p53, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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