Western Blot (WB)
((0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat KLF13 Polyclonal Antibody | anti-KLF13 antibody
Goat anti-KLF13 (aa45-58) Antibody
Gene Names
KLF13; BTEB3; FKLF2; NSLP1; RFLAT1; RFLAT-1
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Pig, Cow
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
KLF13; Polyclonal Antibody; Goat anti-KLF13 (aa45-58) Antibody; Goat Anti-RFLAT / KLF13 Antibody; Kruppel-like factor 13; BTEB3; FKLF2; NSLP1; RFLAT-1; RFLAT1; BTE-binding protein 3; Krueppel-like factor 13; RANTES factor of late activated T lymphocytes-1; RANTES factor of late activated T-lymphocytes 1; Sp1 like zinc finger transcription facto; basic transcription element binding protein 3 antibody; basic transcription element-binding protein 3 antibody; BTEB3 antibody; BTE-binding protein 3 antibody; FKLF2 antibody; Krueppel-like factor 13 antibody; Kruppel-like factor 13 antibody; novel Sp1-like zinc finger transcription factor 1 antibody; NSLP1 antibody; RANTES factor of late activated T lymphocytes-1 antibody; RANTES factor of late activated T-lymphocytes 1 antibody; RFLAT1 antibody; RFLAT-1 antibody; Sp1 like zinc finger transcription factor antibody; transcription factor BTEB3 antibody; transcription factor NSLP1 antibody; KLF13 antibody; KLF13 (aa45-58); anti-KLF13 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Pig, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
TLPRVEERRDGKDS
Sequence Length
288
Applicable Applications for anti-KLF13 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Approx 30+28kDa bands observed in Human Heart lysates (calculated MW of 31.2kDa according to NP_057079.2). Recommended concentration: 0.1-0.3ug/ml. The 28kDa is explained by partly proteolysis. Both bands were blocked by the immunnizing peptide.
Western Blot: Approx 30+28kDa bands observed in Human Heart lysates (calculated MW of 31.2kDa according to NP_057079.2). Recommended concentration: 0.1-0.3ug/ml. The 28kDa is explained by partly proteolysis. Both bands were blocked by the immunnizing peptide.
Immunogen
Peptide with sequence C-TLPRVEERRDGKDS, from the internal region of the protein sequence according to NP_057079.2.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31,180 Da
NCBI Official Full Name
Krueppel-like factor 13 isoform 1
NCBI Official Synonym Full Names
Kruppel-like factor 13
NCBI Official Symbol
KLF13
NCBI Official Synonym Symbols
BTEB3; FKLF2; NSLP1; RFLAT1; RFLAT-1
NCBI Protein Information
Krueppel-like factor 13
UniProt Protein Name
Krueppel-like factor 13
Protein Family
UniProt Gene Name
KLF13
UniProt Synonym Gene Names
BTEB3; NSLP1; BTE-binding protein 3; RFLAT-1
UniProt Entry Name
KLF13_HUMAN
NCBI Description
KLF13 belongs to a family of transcription factors that contain 3 classical zinc finger DNA-binding domains consisting of a zinc atom tetrahedrally coordinated by 2 cysteines and 2 histidines (C2H2 motif). These transcription factors bind to GC-rich sequences and related GT and CACCC boxes (Scohy et al., 2000 [PubMed 11087666]).[supplied by OMIM, Mar 2008]
Uniprot Description
KLF13: Represses transcription by binding to the BTE site, a GC-rich DNA element, in competition with the activator SP1. It also represses transcription by interacting with the corepressor Sin3A and HDAC1. Activates RANTES expression in T-cells. Belongs to the Sp1 C2H2-type zinc-finger protein family.
Protein type: C2H2-type zinc finger protein; DNA-binding
Chromosomal Location of Human Ortholog: 15q12
Cellular Component: nucleus
Molecular Function: metal ion binding; protein binding
Biological Process: negative regulation of cell proliferation; negative regulation of erythrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter
Disease: Chromosome 15q13.3 Deletion Syndrome
Research Articles on KLF13
Similar Products
Product Notes
The KLF13 klf13 (Catalog #AAA422783) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-KLF13 (aa45-58) Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's KLF13 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 64000. Western Blot: Approx 30+28kDa bands observed in Human Heart lysates (calculated MW of 31.2kDa according to NP_057079.2). Recommended concentration: 0.1-0.3ug/ml. The 28kDa is explained by partly proteolysis. Both bands were blocked by the immunnizing peptide. Researchers should empirically determine the suitability of the KLF13 klf13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: TLPRVEERRD GKDS. It is sometimes possible for the material contained within the vial of "KLF13, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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