Rabbit anti-Human MOBKL1B Polyclonal Antibody | anti-MOBKL1B antibody

MOBKL1B, CT (Mps One Binder Kinase Activator-like 1B, Mob1 Homolog 1B, MOBK1B, C2orf6, FLJ10788, FLJ11595, MATS1, MOB4B, Protein Mob4B) (MaxLight 405)

Cat. Num. AAA6488339

• Gene Names: MOB1A; MOB1; MATS1; Mob4B; C2orf6; MOBK1B; MOBKL1B
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: MOBKL1B; Polyclonal Antibody; CT (Mps One Binder Kinase Activator-like 1B; Mob1 Homolog 1B; MOBK1B; C2orf6; FLJ10788; FLJ11595; MATS1; MOB4B; Protein Mob4B) (MaxLight 405); MOB1; Mps One Binder Kinase Activator-like 1B (Yeast); anti-MOBKL1B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MOBKL1B.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-MOBKL1B antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human MOBKL1B (KLH).
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MOBKL1B antibody (C-term) in A2058 cell line lysates (35ug/lane). MOBKL1B (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(MOBKL1B Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human Prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MOBKL1B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Western Blot (WB)

(Western blot analysis of MOBKL1B (arrow) using rabbit polyclonal MOBKL1B Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MOBKL1B gene.)

Flow Cytometry (FC/FACS)

(MOBKL1B Antibody (C-term) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-MOBKL1B antibody

Antibodies; Protein Kinases

References

1. Bichsel, S.J., J. Biol. Chem. 279(34), 35228-35235 (2004). 2. Stavridi, E.S., Structure 11(9), 1163-1170 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 148747437
• NCBI GeneID: 55233
• NCBI Accession #: NP_060691
• NCBI GenBank Nucleotide #: NM_018221.4
• UniProt Accession #: Q9H8S9; Q53S34; Q9H3T5; Q9HAI0; Q9NVE2
• Molecular Weight: 27.2 kDa (236aa), confirmed by MALDI-TOF
• NCBI Official Full Name: MOB kinase activator 1A isoform 1
• NCBI Official Synonym Full Names: MOB kinase activator 1A
• NCBI Official Symbol: MOB1A
• NCBI Official Synonym Symbols: MOB1; MATS1; Mob4B; C2orf6; MOBK1B; MOBKL1B
• NCBI Protein Information: MOB kinase activator 1A
• UniProt Protein Name: MOB kinase activator 1A
• UniProt Gene Name: MOB1A
• UniProt Synonym Gene Names: C2orf6; MOB4B; MOBK1B; MOBKL1B; Mob1A
• UniProt Entry Name: MOB1A_HUMAN

NCBI Description

The protein encoded by this gene is a component of the Hippo signaling pathway, which controls organ size and tumor growth by enhancing apoptosis. Loss of the encoded protein results in cell proliferation and cancer formation. The encoded protein is also involved in the control of microtubule stability during cytokinesis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]

Uniprot Description

MOB1A: Activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38 and STK38L. Acts cooperatively with STK3/MST2 to activate STK38. Binds STK38 and STK38L. Interacts with LATS1 and LATS2. Forms a tripartite complex with STK38 and STK3/MST2. Adrenal gland, bone marrow, brain, placenta, prostate, salivary gland, skeletal muscle, testis, thymus, thyroid gland, heart, spinal cord, fetal brain and fetal liver. Belongs to the MOB1/phocein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 2p13.1

Cellular Component: cytosol

Molecular Function: protein binding; metal ion binding