MOBKL1B recombinant protein

MOBKL1B, 1-216aa, Human, His tag, E Coli

Cat. Num. AAA203969

• Gene Names: MOB1A; MOB1; MATS1; Mob4B; C2orf6; MOBK1B; MOBKL1B
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: MOBKL1B; 1-216aa; Human; His tag; E Coli; Mps one binder kinase activator-like 1B; C2orf6; FLJ10788; FLJ11595; MATS1; MOB1; Mob4B; MOBK1B; MOBKL1B recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 0.2M NaCl, 5mM DTT, 30% glycerol
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MSFLFSSRSS KTFKPKKNIP EGSHQYELLK HAEATLGSGN LRQAVMLPEG EDLNEWIAVN TVDFFNQINM LYGTITEFCT EASCPVMSAG PRYEYHWADG TNIKKPIKCS APKYIDYLMT WVQDQLDDET LFPSKIGVPF PKNFMSVAKT ILKRLFRVYA HIYHQHFDSV MQLQEEAHLN TSFKHFIFFV QEFNLIDRRE LAPLQELIEK LGSKDR
• Sequence Length: 216

• Applicable Applications for MOBKL1B recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for MOBKL1B recombinant protein

MOBKL1B, also known as MOB1B, belongs to the MOB1/phocein family. It stimulates the kinase activity of STK38 and STK38L. MOBKL1B binds to and regulate downstream targets such as the NDR-family protein kinases and LATS1 kinase. Therefore, MOBKL1B participates in cell cycle checkpoint control and tumor inhibition. Recombinant human MOBKL1B protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for MOBKL1B recombinant protein

Cancer

References

Lai ZC., et al. (2005) Cell. 120 (5):675-85.; Bichsel SJ., et al. (2004) J Biol Chem. 279 (34):35228-35.;

NCBI and Uniprot Product Information

• NCBI GI #: 148747437
• NCBI GeneID: 55233
• NCBI Accession #: NP_060691
• NCBI GenBank Nucleotide #: NM_018221.4
• UniProt Accession #: Q9H8S9; Q53S34; Q9H3T5; Q9HAI0; Q9NVE2
• Molecular Weight: 27.2 kDa (236aa), confirmed by MALDI-TOF
• NCBI Official Full Name: MOB kinase activator 1A isoform 1
• NCBI Official Synonym Full Names: MOB kinase activator 1A
• NCBI Official Symbol: MOB1A
• NCBI Official Synonym Symbols: MOB1; MATS1; Mob4B; C2orf6; MOBK1B; MOBKL1B
• NCBI Protein Information: MOB kinase activator 1A
• UniProt Protein Name: MOB kinase activator 1A
• UniProt Gene Name: MOB1A
• UniProt Synonym Gene Names: C2orf6; MOB4B; MOBK1B; MOBKL1B; Mob1A

NCBI Description

The protein encoded by this gene is a component of the Hippo signaling pathway, which controls organ size and tumor growth by enhancing apoptosis. Loss of the encoded protein results in cell proliferation and cancer formation. The encoded protein is also involved in the control of microtubule stability during cytokinesis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015]

Uniprot Description

Activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38 and STK38L. Acts cooperatively with STK3/MST2 to activate STK38.