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Western Blot (WB) ((1ug/ml) staining of Daudi lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Goat ZBTB24 Polyclonal Antibody | anti-ZBTB24 antibody

Goat anti-ZBTB24 (aa232-245) Antibody

Gene Names
ZBTB24; BIF1; ICF2; PATZ2; ZNF450
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
ZBTB24; Polyclonal Antibody; Goat anti-ZBTB24 (aa232-245) Antibody; zinc finger and BTB domain containing 24; BIF1; ICF2; PATZ2; ZNF450; OTTHUMP00000016968; POZ (BTB) and AT hook containing zinc finger 2; zinc finger and BTB domain-containing protein 24; zinc finger protein 450; BIF1 antibody; ICF2 antibody; OTTHUMP00000016968 antibody; PATZ2 antibody; POZ (BTB) and AT hook containing zinc finger 2 antibody; zinc finger and BTB domain containing 24 antibody; zinc finger and BTB domain-containing protein 24 antibody; zinc finger protein 450 antibody; ZNF450 antibody; ZBTB24 antibody; ZBTB24 (aa232-245); anti-ZBTB24 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
PVEKDENYDPKTED
Sequence Length
697
Applicable Applications for anti-ZBTB24 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Approx 80kDa band observed in lysates of cell line Daudi (calculated MW of 78.3kDa according to NP_055612.2). Recommended concentration: 1-3ug/ml.
Immunogen
Peptide with sequence C-PVEKDENYDPKTED, from the internal region of the protein sequence according to NP_055612.2; NP_001157785.1.
Epitope
Internal region
Note
This antibody is expected to recognize both reported isoforms (NP_055612.2; NP_001157785.1).
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((1ug/ml) staining of Daudi lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((1ug/ml) staining of Daudi lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37,620 Da
NCBI Official Full Name
zinc finger and BTB domain-containing protein 24 isoform 1
NCBI Official Synonym Full Names
zinc finger and BTB domain containing 24
NCBI Official Symbol
ZBTB24
NCBI Official Synonym Symbols
BIF1; ICF2; PATZ2; ZNF450
NCBI Protein Information
zinc finger and BTB domain-containing protein 24
UniProt Protein Name
Zinc finger and BTB domain-containing protein 24
UniProt Gene Name
ZBTB24
UniProt Synonym Gene Names
KIAA0441; ZNF450
UniProt Entry Name
ZBT24_HUMAN

NCBI Description

This gene encodes a protein similar to a protein in rodents which is induced by bone morphogenic protein 2 in vitro. [provided by RefSeq, Aug 2011]

Uniprot Description

ZBTB24: May be involved in BMP2-induced transcription. Defects in ZBTB24 are the cause of immunodeficiency- centromeric instability-facial anomalies syndrome type 2 (ICF2). A rare disorder characterized by a variable immunodeficiency resulting in recurrent infections, facial anomalies, and branching of chromosomes 1, 9, and 16. Other variable symptoms include growth retardation, failure to thrive, and psychomotor retardation. Laboratory studies show limited hypomethylation of DNA in a small fraction of the genome in some, but not all, patients. Belongs to the krueppel C2H2-type zinc-finger protein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: nucleus

Molecular Function: DNA binding; metal ion binding; protein binding

Biological Process: hemopoietic progenitor cell differentiation; regulation of transcription, DNA-dependent; transcription, DNA-dependent

Disease: Immunodeficiency-centromeric Instability-facial Anomalies Syndrome 2

Research Articles on ZBTB24

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Product Notes

The ZBTB24 zbtb24 (Catalog #AAA423011) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ZBTB24 (aa232-245) Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZBTB24 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 8000. Western Blot: Approx 80kDa band observed in lysates of cell line Daudi (calculated MW of 78.3kDa according to NP_055612.2). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the ZBTB24 zbtb24 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: PVEKDENYDP KTED. It is sometimes possible for the material contained within the vial of "ZBTB24, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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