Rabbit Mlx Polyclonal Antibody | anti-Mlx antibody

Mlx (Q136) Polyclonal Antibody

Cat. Num. AAA3002217

• Gene Names: MLX; TF4; MAD7; MXD7; TCFL4; bHLHd13
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Mlx; Polyclonal Antibody; Mlx (Q136) Polyclonal Antibody; Max-like protein X; Class D basic helix-loop-helix protein 13; bHLHd13; Max-like bHLHZip protein; Protein BigMax; Transcription factor-like protein 4; MLX; BHLHD13; TCFL4; anti-Mlx antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Mlx (Q136) polyclonal antibody detects endogenous levels of Mlx protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 298

• Applicable Applications for anti-Mlx antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 102-150 of Human Mlx.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Testing Data

Related Product Information for anti-Mlx antibody

Max is a nuclear localized bHLH-Zip protein that forms homodimers or heterodimers with Myc family members, including Myc, Mad 1, Mad 3, Mad 4, Mxi1 and Mnt (or Rox). These dimers bind to the E-box sequence CACGTG in order to regulate cell growth, proliferation and apoptosis. Mlx (Max-like protein X) is a bHLH-Zip protein that is structurally and functionally related to Max. Like Max, Mlx is broadly expressed in many tissues and has a long half-life. Mlx also forms homodimers or heterodimers with members of the Myc family, specifically Mad 1, Mad 4 and Rox, and members of the Mondo family, to repress or activate transcription from CACGTG E-boxes. MondoA forms weak homodimers and preferentially forms heterodimers with Mlx. The MondoA/Mlx complex is primarily localized to the cytoplasm, but will translocate to the nucleus in response to leptomycin B. Mlx can also dimerize with WBSCR14, a protein involved in Williams-Beuren syndrome (WBS), to repress E-box transcription, which provides further evidence that Mlx is a critical element in a transcription factor network.

NCBI and Uniprot Product Information

• NCBI GI #: 24586667
• NCBI GeneID: 6945
• NCBI Accession #: NP_733752.1
• NCBI GenBank Nucleotide #: NP_733752.1
• UniProt Accession #: Q9UH92
• Molecular Weight: ~ 33kDa
• NCBI Official Full Name: max-like protein X isoform gamma
• NCBI Official Synonym Full Names: MAX dimerization protein MLX
• NCBI Official Symbol: MLX
• NCBI Official Synonym Symbols: TF4; MAD7; MXD7; TCFL4; bHLHd13
• NCBI Protein Information: max-like protein X
• UniProt Protein Name: Max-like protein X
• Protein Family: Max-like protein
• UniProt Gene Name: MLX
• UniProt Synonym Gene Names: BHLHD13; TCFL4; bHLHd13
• UniProt Entry Name: MLX_HUMAN

NCBI Description

The product of this gene belongs to the family of basic helix-loop-helix leucine zipper (bHLH-Zip) transcription factors. These factors form heterodimers with Mad proteins and play a role in proliferation, determination and differentiation. This gene product may act to diversify Mad family function by its restricted association with a subset of the Mad family of transcriptional repressors, namely, Mad1 and Mad4. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

MLX: Transcription regulator. Forms a sequence-specific DNA- binding protein complex with MAD1, MAD4, MNT, WBSCR14 and MLXIP which recognizes the core sequence 5'-CACGTG-3'. The TCFL4-MAD1, TCFL4-MAD4, TCFL4-WBSCR14 complexes are transcriptional repressors. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 17q21.1

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; transcription factor activity; transcription factor binding

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; energy reserve metabolic process; positive regulation of cellular metabolic process; nucleocytoplasmic transport; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent