Western Blot (WB)
(Western blot analysis of RASEF expression in K-562 cell lysate at 25ug/lane., The lane on the left is treated with the antigen-specific peptide.)
Rabbit anti-Human, Mouse RASEF Polyclonal Antibody | anti-RASEF antibody
RASEF Antibody
Gene Names
RASEF; RAB45
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
RASEF; Polyclonal Antibody; RASEF Antibody; FLJ31614; RAB45; RAB45 member RAS oncogene family; RAS and EF hand domain containing; RAS and EF hand domain containing protein; Ras related protein Rab 45; anti-RASEF antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
RASEF antibody detects endogenous levels of total RASEF
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
740
Applicable Applications for anti-RASEF antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide
Tissue Specificity
Down-regulated in cutaneous melanoma cells but not in breast cancer cells.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Dog
Similarity
Pig (82%), Bovine (91%), Horse (82%), Sheep (91%), Dog (91%)
Conjugation
Unconjugated
Western Blot (WB)
(Western blot analysis of RASEF expression in K-562 cell lysate at 25ug/lane., The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of RASEF expression in K-562 cell lysate at 25ug/lane., The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-RASEF antibody
Function: Binds predominantly GDP, and also GTP.
Subunit Structure: Homodimer.
Similarity: Belongs to the small GTPase superfamily. Rab family.
Subunit Structure: Homodimer.
Similarity: Belongs to the small GTPase superfamily. Rab family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 83 kDa
Predicted: 83 kDa
Predicted: 83 kDa
NCBI Official Full Name
ras and EF-hand domain-containing protein
NCBI Official Synonym Full Names
RAS and EF-hand domain containing
NCBI Official Symbol
RASEF
NCBI Official Synonym Symbols
RAB45
NCBI Protein Information
ras and EF-hand domain-containing protein
UniProt Protein Name
Ras and EF-hand domain-containing protein
Protein Family
UniProt Gene Name
RASEF
UniProt Synonym Gene Names
RAB45
NCBI Description
This gene is a member of the Rab family of GTPases that are involved in regulation of membrane traffic. The encoded protein contains an N-terminal EF-hand domain, a coiled-coil motif and a C-terminal Rab domain. A potential role as tumor suppressor has been indicated for this gene. [provided by RefSeq, Nov 2012]
Uniprot Description
Binds predominantly GDP, and also GTP.
Research Articles on RASEF
Similar Products
Product Notes
The RASEF rasef (Catalog #AAA9604160) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RASEF Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's RASEF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the RASEF rasef for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RASEF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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