Mouse anti-Human MLX Monoclonal Antibody | anti-MLX antibody

MLX Mouse Monoclonal Antibody [Clone PCRP-MLX-1G8]

Cat. Num. AAA4382264

• Gene Names: MLX; MAD7; MXD7; TCFL4; bHLHd13
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
• Synonyms: MLX; Monoclonal Antibody; MLX Mouse Monoclonal Antibody [Clone PCRP-MLX-1G8]; bHLHd13; Class D basic helix-loop-helix protein 13; MAD7; Max-like bHLHZip protein; Max-like protein X; MLXIP; MONDOA; MXD7; Protein BigMax; Protein Mlx; Transcription factor-like protein 4 (TCFL4); anti-MLX antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse/IgG2b
• Clone Number: PCRP-MLX-1G8
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0. 05% BSA & 0. 05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Concentration: Purified Ab with BSA and Azide at 200ug/ml; OR; Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)

• Applicable Applications for anti-MLX antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: FC/FACS: 1-2ug/million cells; IF: 1-2ug/ml; WB: 1-2ug/ml
• Immunogen: Recombinant full-length human MLX protein
• Cellular Localization: Nucleus and cytoplasm
• Positive Control: HeLa or MCF7 cells.
• Hu-chromosome Location: 17q21.2
• Unigene: 383019 & 714749
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C; Antibody without azide: store at -20 to -80 degree C; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Flow Cytometry (FC/FACS)

(Flow Cytometric Analysis of PFA-fixed HeLa cells. MLX Mouse Monoclonal Antibody (PCRP-MLX-1G8) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red). )

Related Product Information for anti-MLX antibody

Max is a nuclear localized bHLH-Zip protein that forms homodimers or heterodimers with Myc family members, including Myc, Mad1, Mad3, Mad4, Mxi1 and Mnt (or Rox). These dimers bind to the E-box sequence CACGTG in order to regulate cell growth, proliferation and apoptosis. Mlx (Max-like protein X) is a bHLH-Zip protein that is structurally and functionally related to Max. Like Max, Mlx is broadly expressed in many tissues and has a long half-life. Mlx also forms homodimers or heterodimers with members of the Myc family, specifically Mad1, Mad4 and Rox, and members of the Mondo family, to repress or activate transcription from CACGTG E-boxes. MondoA forms weak homodimers and preferentially forms heterodimers with Mlx. The MondoA/Mlx complex is primarily localized to the cytoplasm, but will translocate to the nucleus in response to leptomycin B. Mlx can also dimerize with WBSCR14, a protein involved in Williams-Beuren syndrome (WBS), to repress E-box transcription, which provides further evidence that Mlx is a critical element in a transcription factor network.

NCBI and Uniprot Product Information

• NCBI GI #: 24586667
• NCBI GeneID: 6945
• NCBI Accession #: NP_733752.1
• NCBI GenBank Nucleotide #: NM_170607.2
• UniProt Accession #: Q9UH92; Q53XM6; Q96FL2; Q9H2V0; Q9H2V1; Q9H2V2; Q9NXN3; A8K2J3; B2RAV8; B2RD73
• Molecular Weight: 33,300 Da
• NCBI Official Full Name: max-like protein X isoform gamma
• NCBI Official Synonym Full Names: MLX, MAX dimerization protein
• NCBI Official Symbol: MLX
• NCBI Official Synonym Symbols: MAD7; MXD7; TCFL4; bHLHd13
• NCBI Protein Information: max-like protein X; BigMax protein; MAX-like bHLHZIP protein; transcription factor-like protein 4; class D basic helix-loop-helix protein 13
• UniProt Protein Name: Max-like protein X
• Protein Family: Max-like protein
• UniProt Gene Name: MLX
• UniProt Synonym Gene Names: BHLHD13; TCFL4; bHLHd13
• UniProt Entry Name: MLX_HUMAN

NCBI Description

The product of this gene belongs to the family of basic helix-loop-helix leucine zipper (bHLH-Zip) transcription factors. These factors form heterodimers with Mad proteins and play a role in proliferation, determination and differentiation. This gene product may act to diversify Mad family function by its restricted association with a subset of the Mad family of transcriptional repressors, namely, Mad1 and Mad4. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

MLX: Transcription regulator. Forms a sequence-specific DNA- binding protein complex with MAD1, MAD4, MNT, WBSCR14 and MLXIP which recognizes the core sequence 5'-CACGTG-3'. The TCFL4-MAD1, TCFL4-MAD4, TCFL4-WBSCR14 complexes are transcriptional repressors. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 17q21.1

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; transcription factor activity; transcription factor binding

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; energy reserve metabolic process; positive regulation of cellular metabolic process; nucleocytoplasmic transport; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent