Rabbit MLK1/2 Polyclonal Antibody | anti-MLK1/2 antibody
Phospho-MLK1/2 (Thr312/Thr266) Antibody
Gene Names
MAP3K9; MLK1; MEKK9; PRKE1
Reactivity
Human, Mouse, Rat
Applications
ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
MLK1/2; Polyclonal Antibody; Phospho-MLK1/2 (Thr312/Thr266) Antibody; M3K9_HUMAN; Map3k9; MEKK9; Mitogen activated protein kinase kinase kinase 9; Mitogen-activated protein kinase kinase kinase 9; Mixed lineage kinase 1 (tyr and ser/thr specificity); Mixed lineage kinase 1; MLK1; PRKE1; M3K10_HUMAN; MAP3K 10; Map3k10; MEKK10; Mitogen activated protein kinase kinase kinase 10; Mitogen-activated protein kinase kinase kinase 10; Mixed lineage kinase 2; MKN28 derived nonreceptor type serine/threonine kinase; MKN28 kinase; MLK 2; MST; Protein kinase MST; anti-MLK1/2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-MLK1/2 (Thr312/Thr266) Antibody detects endogenous levels of MLK1/2 only when phosphorylated at Thr312/266.
Tissue Specificity: Expressed in brain and skeletal muscle.
Tissue Specificity: Expressed in brain and skeletal muscle.
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-MLK1/2 antibody
Peptide ELISA (EIA)
Application Notes
Peptide ELISA: 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human MLK1/2 around the phosphorylation site of Thr312/266.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation.
Subunit Structure
Homodimer. Interacts with SH3RF2.
Similarity
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Related Product Information for anti-MLK1/2 antibody
Activates the JUN N-terminal pathway.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
123,373 Da
NCBI Official Full Name
mitogen-activated protein kinase kinase kinase 9 isoform 2
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase kinase 9
NCBI Official Symbol
MAP3K9
NCBI Official Synonym Symbols
MLK1; MEKK9; PRKE1
NCBI Protein Information
mitogen-activated protein kinase kinase kinase 9; mixed lineage kinase 1 (tyr and ser/thr specificity)
UniProt Protein Name
Mitogen-activated protein kinase kinase kinase 9
UniProt Gene Name
MAP3K9
UniProt Synonym Gene Names
MLK1; PRKE1
UniProt Entry Name
M3K9_HUMAN
Uniprot Description
MLK1: a TKL kinase of the MLK family. A dual leucine zipper kinase. Expressed in neuronal cells and may act between Rac1/Cdc42 and MKK4 and -7 in death signaling.
Protein type: EC 2.7.11.25; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; TKL group; MLK family; MLK subfamily
Chromosomal Location of Human Ortholog: 14q24.2
Molecular Function: MAP kinase kinase activity; protein serine/threonine kinase activity; protein binding; protein homodimerization activity; JUN kinase kinase kinase activity; protein-tyrosine kinase activity; ATP binding
Biological Process: peptidyl-tyrosine phosphorylation; regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of apoptosis; apoptosis; protein amino acid autophosphorylation; protein amino acid phosphorylation; activation of JNK activity; activation of JNKK activity
Research Articles on MLK1/2
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Product Notes
The MLK1/2 map3k9 (Catalog #AAA9612698) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-MLK1/2 (Thr312/Thr266) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MLK1/2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA). Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the MLK1/2 map3k9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MLK1/2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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