Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human brain tissue using MLK1/2 (Phospho-Thr312/266) antibody (left)or the same antibody preincubated with blocking peptide (right).)
Rabbit anti-Human, Mouse MLK1/2 Polyclonal Antibody | anti-MLK1/2 antibody
MLK1/2 (Phospho-Thr312/266) Antibody
Gene Names
MAP3K9; MLK1; MEKK9; PRKE1
Reactivity
Human, Mouse
Applications
Immunohistochemistry
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Synonyms
MLK1/2; Polyclonal Antibody; MLK1/2 (Phospho-Thr312/266) Antibody; M3K9; PRKE1; mixed-lineage protein kinase 1; anti-MLK1/2 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of MLK1/2 only when phosphorylated at threonine 312/266.
Purity/Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
1104
Applicable Applications for anti-MLK1/2 antibody
Immunohistochemistry (IHC)
Application Notes
Immunohistochemistry: 1:50~1:100
Immunogen Type
Peptide-KLH
Immunogen Description
Peptide sequence around phosphorylation site of threonine 312/266(A-G-T(p)-Y-A) derived from Human MLK1/2.
Modification
Phospho-Thr312/266
Target Name
MLK1/2
Preparation and Storage
Store at -20 degree C/1 year
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human brain tissue using MLK1/2 (Phospho-Thr312/266) antibody (left)or the same antibody preincubated with blocking peptide (right).)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human brain tissue using MLK1/2 (Phospho-Thr312/266) antibody (left)or the same antibody preincubated with blocking peptide (right).)
Related Product Information for anti-MLK1/2 antibody
The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase has been shown to activate MAPK8/JNK and MKK4/SEK1, and this kinase itself can be phoshorylated, and thus activated by JNK kinases. This kinase functions preferentially on the JNK signaling pathway, and is reported to be involved in nerve growth factor (NGF) induced neuronal apoptosis.Durkin J.T., Biochemistry 43:16348-16355(2004).The MGC Project Team; Genome Res. 14:2121-2127(2004).Dorow D.S.,Nature 446:153-158(2007).
Product Categories/Family for anti-MLK1/2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
123,373 Da
NCBI Official Full Name
mitogen-activated protein kinase kinase kinase 9 isoform 2
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase kinase 9
NCBI Official Symbol
MAP3K9
NCBI Official Synonym Symbols
MLK1; MEKK9; PRKE1
NCBI Protein Information
mitogen-activated protein kinase kinase kinase 9
UniProt Protein Name
Mitogen-activated protein kinase kinase kinase 9
UniProt Gene Name
MAP3K9
UniProt Synonym Gene Names
MLK1; PRKE1
UniProt Entry Name
M3K9_HUMAN
Uniprot Description
MLK1: a TKL kinase of the MLK family. A dual leucine zipper kinase. Expressed in neuronal cells and may act between Rac1/Cdc42 and MKK4 and -7 in death signaling.
Protein type: Protein kinase, TKL; Kinase, protein; EC 2.7.11.25; Protein kinase, Ser/Thr (non-receptor); TKL group; MLK family; MLK subfamily
Chromosomal Location of Human Ortholog: 14q24.2
Molecular Function: MAP kinase kinase activity; protein serine/threonine kinase activity; protein binding; JUN kinase kinase kinase activity; protein homodimerization activity; protein-tyrosine kinase activity; ATP binding
Biological Process: peptidyl-tyrosine phosphorylation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; apoptosis; positive regulation of apoptosis; protein amino acid autophosphorylation; protein amino acid phosphorylation; activation of JNK activity; activation of JNKK activity
Research Articles on MLK1/2
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Product Notes
The MLK1/2 map3k9 (Catalog #AAA9414936) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MLK1/2 (Phospho-Thr312/266) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MLK1/2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC). Immunohistochemistry: 1:50~1:100. Researchers should empirically determine the suitability of the MLK1/2 map3k9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MLK1/2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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