Rabbit Mitofusin1 Polyclonal Antibody | anti-MFN1 antibody

Mitofusin1 Rabbit pAb

Cat. Num. AAA4757670

• Gene Names: MFN1; hfzo1; hfzo2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Affinity purified
• Synonyms: Mitofusin1; Polyclonal Antibody; Mitofusin1 Rabbit pAb; Mitofusin-1; mitofusin 1; hfzo1; hfzo2; anti-MFN1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Detected in kidney and heart (at protein level) (PubMed:12759376). Ubiquitous (PubMed:11950885, PubMed:12759376). Expressed at slightly higher level in kidney and heart (PubMed:12759376). Isoform 2 may be overexpressed in some tumors, such as lung cancers (PubMed:11751411).
• Purity/Purification: Affinity purified
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

• Applicable Applications for anti-MFN1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IF: 1:50-1:200
• Immunogen: Recombinant protein of human MFN1 (NP_284941.2).
• Conjugation: Unconjugated
• Molecular Function: Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion (PubMed:12475957, PubMed:12759376, PubMed:27920125, PubMed:28114303). Membrane clustering requires GTPase activity (PubMed:27920125). It may involve a major rearrangement of the coiled coil domains (PubMed:27920125, PubMed:28114303). Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events (PubMed:12475957, PubMed:12759376). Overexpression induces the formation of mitochondrial networks (in vitro) (PubMed:12759376). Has low GTPase activity (PubMed:27920125, PubMed:28114303).
• Modification: Unmodified
• Cell Position: Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot - MFN1 Polyclonal Antibody.Western blot analysis of extracts of various cell lines, using MFN1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Exposure time: 5s.)

Related Product Information for anti-MFN1 antibody

The protein encoded by this gene is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). They are mitochondrial membrane proteins that interact with each other to facilitate mitochondrial targeting. [provided by RefSeq, Jul 2008]

Product Categories/Family for anti-MFN1 antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 45269137
• NCBI GeneID: 55669
• NCBI Accession #: NP_284941
• NCBI GenBank Nucleotide #: NM_033540.2
• UniProt Accession #: Q8IWA4; O15323; O60639; Q9BZB5; Q9NWQ2; B2RAR1; D3DNR6
• Molecular Weight: Predicted: 82 kDa; ; Observed: 83 kDa
• NCBI Official Full Name: mitofusin-1
• NCBI Official Synonym Full Names: mitofusin 1
• NCBI Official Symbol: MFN1
• NCBI Official Synonym Symbols: hfzo1; hfzo2
• NCBI Protein Information: mitofusin-1; fzo homolog; transmembrane GTPase MFN1; putative transmembrane GTPase; mitochondrial transmembrane GTPase FZO-2; mitochondrial transmembrane GTPase Fzo-1
• UniProt Protein Name: Mitofusin-1
• Protein Family: Mitofusin
• UniProt Gene Name: MFN1
• UniProt Entry Name: MFN1_HUMAN

NCBI Description

The protein encoded by this gene is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). They are mitochondrial membrane proteins that interact with each other to facilitate mitochondrial targeting. [provided by RefSeq, Jul 2008]

Uniprot Description

MFN1: Essential transmembrane GTPase, which mediates mitochondrial fusion. Fusion of mitochondria occurs in many cell types and constitutes an important step in mitochondria morphology, which is balanced between fusion and fission. MFN1 acts independently of the cytoskeleton. Overexpression induces the formation of mitochondrial networks. Belongs to the mitofusin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; Mitochondrial; Membrane protein, multi-pass; Membrane protein, integral; EC 3.6.5.-

Chromosomal Location of Human Ortholog: 3q26.33

Cellular Component: mitochondrial outer membrane; integral to membrane

Molecular Function: GTPase activity; protein binding; GTP binding

Biological Process: mitochondrial fusion; metabolic process