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Western Blot (WB) (Western Blot: Sample: Recombinant MRPL1, Mouse.)

Rabbit anti-Mouse Mitochondrial Ribosomal Protein L1 Polyclonal Antibody | anti-MRPL1 antibody

Mitochondrial Ribosomal Protein L1 (MRPL1) Polyclonal Antibody

Gene Names
Mrpl1; L1mt; AI462572; 2410002L03Rik; 5830418D04Rik
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Mitochondrial Ribosomal Protein L1; Polyclonal Antibody; Mitochondrial Ribosomal Protein L1 (MRPL1) Polyclonal Antibody; MRP-L1; BM022; L1mt; 39S Ribosomal Protein L1; Mitochondrial; anti-MRPL1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against MRPL1. It has been selected for its ability to recognize MRPL1 in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Concentration
0.57mg/ml (varies by lot)
Sequence Length
325
Applicable Applications for anti-MRPL1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Cross Reactivity
Mouse
Organism Species
Mouse
Fragment
MRPL1 (Glu143~Ala336)
Quality Control
Content: The quality control contains recombinant MRPL1 (Glu143~Ala336) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2067237
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant MRPL1, Mouse.)

Western Blot (WB) (Western Blot: Sample: Recombinant MRPL1, Mouse.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Stomach Tissue.)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Mouse Stomach Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Stomach Tissue))

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Mouse Stomach Tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37,597 Da
NCBI Official Full Name
39S ribosomal protein L1, mitochondrial isoform 1
NCBI Official Synonym Full Names
mitochondrial ribosomal protein L1
NCBI Official Symbol
Mrpl1
NCBI Official Synonym Symbols
L1mt; AI462572; 2410002L03Rik; 5830418D04Rik
NCBI Protein Information
39S ribosomal protein L1, mitochondrial
UniProt Protein Name
39S ribosomal protein L1, mitochondrial
Protein Family
UniProt Gene Name
Mrpl1
UniProt Synonym Gene Names
L1mt; MRP-L1

Uniprot Description

MRPL1: a mitochondrial ribosomal protein encoded by a nuclear gene that helps in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L1 ribosomal protein family. [provided by RefSeq, Jul 2008]

Protein type: Mitochondrial; RNA-binding

Chromosomal Location of Human Ortholog: 5|5 E3

Cellular Component: intracellular ribonucleoprotein complex; large ribosomal subunit; mitochondrial large ribosomal subunit; mitochondrion; ribosome

Molecular Function: RNA binding; structural constituent of ribosome

Biological Process: cleavage involved in rRNA processing; maturation of LSU-rRNA; rRNA pseudouridine synthesis; snRNA pseudouridine synthesis; translation

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Product Notes

The MRPL1 mrpl1 (Catalog #AAA2032687) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Mitochondrial Ribosomal Protein L1 (MRPL1) Polyclonal Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Mitochondrial Ribosomal Protein L1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the MRPL1 mrpl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mitochondrial Ribosomal Protein L1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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