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Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Testis Tissue; Lane2: Rat Testis Tissue.)
Rabbit anti-Mouse Sperm Protein 17 Polyclonal Antibody | anti-Sp17 antibody
Sperm Protein 17 (Sp17) Polyclonal Antibody
Gene Names
Spa17; Sp17
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Sperm Protein 17; Polyclonal Antibody; Sperm Protein 17 (Sp17) Polyclonal Antibody; SPA17; SP17; SP17-1; CT22; Sperm Autoantigenic Protein 17; Cancer/Testis Antigen 22; anti-Sp17 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against Sp17. It has been selected for its ability to recognize Sp17 in immunohistochemical staining and western blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
Concentration
500ug/mL (varies by lot)
Applicable Applications for anti-Sp17 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunoglobulin Type
IgG
Conjugate
No Conjugate
Cross Reactivity
Mouse, Rat
Organism Species
Mouse
Fragment
Sp17 (Met1~Asn149)
Quality Control
Content: The quality control contains recombinant Sp17 (Met1~Asn149) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2060909
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Testis Tissue; Lane2: Rat Testis Tissue.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Mouse Testis Tissue; Lane2: Rat Testis Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant Sp17, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant Sp17, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Testis Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Testis Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Testis Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Testis Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
17,296 Da
NCBI Official Full Name
sperm surface protein Sp17
NCBI Official Synonym Full Names
sperm autoantigenic protein 17
NCBI Official Symbol
Spa17
NCBI Official Synonym Symbols
Sp17
NCBI Protein Information
sperm surface protein Sp17
UniProt Protein Name
Sperm surface protein Sp17
UniProt Gene Name
Spa17
UniProt Synonym Gene Names
Sp17
Uniprot Description
SPA17: Sperm surface zona pellucida binding protein. Helps to bind spermatozoa to the zona pellucida with high affinity. Might function in binding zona pellucida and carbohydrates.
Protein type: Cancer Testis Antigen (CTA)
Chromosomal Location of Human Ortholog: 9|9 A4
Cellular Component: cilium; cytoplasm; external side of plasma membrane; membrane
Biological Process: binding of sperm to zona pellucida
Research Articles on Sp17
Similar Products
Product Notes
The Sp17 spa17 (Catalog #AAA2032305) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Sperm Protein 17 (Sp17) Polyclonal Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Sperm Protein 17 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the Sp17 spa17 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Sperm Protein 17, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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