Rabbit anti-Human Mitochondrial Antiviral Signaling Protein Polyclonal Antibody | anti-MAVS antibody

Mitochondrial Antiviral Signaling Protein (MAVS, VISA)

Cat. Num. AAA610711

• Gene Names: MAVS; IPS1; VISA; IPS-1; CARDIF; MGC3260; FLJ27482; FLJ31698; FLJ35386; FLJ38051; FLJ41962; KIAA1271; DKFZp547C224; DKFZp666M015
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: Mitochondrial Antiviral Signaling Protein; Polyclonal Antibody; Mitochondrial Antiviral Signaling Protein (MAVS; VISA); Anti -Mitochondrial Antiviral Signaling Protein (MAVS; anti-MAVS antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Detects endogenous levels of total human MAVS protein. Bands detected at 52 and 75kD.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA and 50% glycerol.

• Applicable Applications for anti-MAVS antibody: ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: Dilution: Western Blot: 1:1000; Immunofluorescence (IF-IC): 1:50
• Immunogen: Synthetic peptide (KLH-coupled) corresponding to residues at the carboxyl terminus of human MAVS
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from LNCaP, MCF-7, and HT29 cell lines using MBS610711)

Western Blot (WB)

(Western blot analysis of extracts from HeLa cells, mock-transfected or transfected with human MAVS, using MBS610711)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of MCF-7 cells using MBS610711 (green) showing colocalization with mitochondria that have been labeled with MitoTracker® Red CMXRos (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).)

Related Product Information for anti-MAVS antibody

The mitochondrial antiviral signaling protein (MAVS, VISA) contributes to innate immunity by triggering IRF-3 and NF- B activation in response to viral infection, leading to the production of IFN- (1). The MAVS protein contains an N-terminal CARD domain and a C-terminal mitochondrial transmembrane domain. The MAVS adaptor protein plays a critical and specific role in viral defenses (2). MAVS acts downstream of the RIG-I RNA helicase and viral RNA sensor, leading to the recruitment of IKK, TRIF and TRAF6 (3,4). Some viruses have evolved strategies to circumvent these innate defenses by using proteases that cleave MAVS to prevent its mitochondrial localization (5,6).

Product Categories/Family for anti-MAVS antibody

Antibodies; Abs to Mitochondrial Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 83776598
• NCBI GeneID: 57506
• NCBI Accession #: NP_065797.2
• NCBI GenBank Nucleotide #: NM_020746.4
• UniProt Accession #: Q7Z434; Q2HWT5; Q3I0Y2; Q5T7I6; Q86VY7; Q9H1H3; Q9H4Y1; Q9H8D3; Q9ULE9
• Molecular Weight: 75kD, 52kD
• NCBI Official Full Name: mitochondrial antiviral-signaling protein isoform 1
• NCBI Official Synonym Full Names: mitochondrial antiviral signaling protein
• NCBI Official Symbol: MAVS
• NCBI Official Synonym Symbols: IPS1; VISA; IPS-1; CARDIF; MGC3260; FLJ27482; FLJ31698; FLJ35386; FLJ38051; FLJ41962; KIAA1271; DKFZp547C224; DKFZp666M015
• NCBI Protein Information: mitochondrial antiviral-signaling protein; OTTHUMP00000030141; IFN-B promoter stimulator 1; CARD adaptor inducing IFN-beta; virus-induced signaling adaptor; virus-induced-signaling adapter; CARD adapter inducing interferon beta; mitochondrial anti-viral signaling protein; putative NF-kappa-B-activating protein 031N; interferon beta promoter stimulator protein 1
• UniProt Protein Name: Mitochondrial antiviral-signaling protein
• Protein Family: Mitochondrial antiviral-signaling protein
• UniProt Gene Name: MAVS
• UniProt Synonym Gene Names: IPS1; KIAA1271; VISA
• UniProt Entry Name: MAVS_HUMAN

NCBI Description

Double-stranded RNA viruses are recognized in a cell type-dependent manner by the transmembrane receptor TLR3 (MIM 603029) or by the cytoplasmic RNA helicases MDA5 (MIM 606951) and RIGI (ROBO3; MIM 608630). These interactions initiate signaling pathways that differ in their initial steps but converge in the activation of the protein kinases IKKA (CHUK; MIM 600664) and IKKB (IKBKB; MIM 603258), which activate NFKB (see MIM 164011), or TBK1 (MIM 604834) and IKKE (IKBKE; MIM 605048), which activate IRF3 (MIM 603734). Activated IRF3 and NFKB induce transcription of IFNB (IFNB1; MIM 147640). For the TLR3 pathway, the intermediary molecule before the pathways converge is the cytoplasmic protein TRIF (TICAM1; MIM 607601). For RIGI, the intermediary protein is mitochondria-bound IPS1 (Sen and Sarkar, 2005 [PubMed 16239922]).[supplied by OMIM]

Uniprot Description

MAVS: Required for innate immune defense against viruses. Acts downstream of DDX58/RIG-I and IFIH1/MDA5, which detect intracellular dsRNA produced during viral replication, to coordinate pathways leading to the activation of NF-kappa-B, IRF3 and IRF7, and to the subsequent induction of antiviral cytokines such as IFN-beta and RANTES (CCL5). May activate the same pathways following detection of extracellular dsRNA by TLR3. May protect cells from apoptosis. Interacts with DDX58/RIG-I, IFIH1/MDA5, TRAF2 and TRAF6. May interact with IRF3, FADD, RIPK1, CHUK and IKBKB. Does not interact with TBK1. Interacts with and is cleaved by HCV and hepatitis GB virus B NS3/4A proteases. Interacts with and is cleaved by HHAV protein 3ABC. Interacts with NLRX1. Interaction with NLRX1 requires the CARD domain. Interacts with PSMA7. Interacts with TRAFD1. Interacts (via C-terminus) with PCBP2 in a complex containing MAVS/IPS1, PCBP2 and ITCH. Interacts with CYLD. Interacts with SRC. Interacts with DHX58/LGP2 and IKBKE. Present in T-cells, monocytes, epithelial cells and hepatocytes. Ubiquitously expressed, with highest levels in heart, skeletal muscle, liver, placenta and peripheral blood leukocytes. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: peroxisomal membrane; mitochondrial outer membrane; mitochondrion; mitochondrial membrane; integral to membrane

Molecular Function: protein binding; signal transducer activity; CARD domain binding; protein kinase binding

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; positive regulation of protein import into nucleus, translocation; signal transduction; positive regulation of tumor necrosis factor production; positive regulation of interleukin-8 production; negative regulation of viral genome replication; defense response to bacterium; positive regulation of transcription factor import into nucleus; positive regulation of interferon-beta production; innate immune response; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; positive regulation of defense response to virus by host; activation of innate immune response; defense response to virus; negative regulation of interferon type I production; positive regulation of interferon-alpha production