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Western Blot (WB) (Western blot analysis of anti-MINA (C-term) Pab pre-incubated with and without blocking peptide (BP)(catlog #:BP1033b) in Jurkat cell line lysate. MINA(C-term)(arrow) was detected using the purified Pab.)

Rabbit anti-Human MINA Polyclonal Antibody | anti-MINA antibody

MINA, CT (Protein Mina53, MYC-induced Nuclear Antigen, Mineral Dust-induced Gene Protein 2, MINA, MDIG, Mina53, Nucleolar Protein 52, MINA53, NO52) (PE)

Gene Names
RIOX2; ROX; MDIG; MINA; NO52; JMJD10; MINA53
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein G Affinity Chromatography.
Synonyms
MINA; Polyclonal Antibody; CT (Protein Mina53; MYC-induced Nuclear Antigen; Mineral Dust-induced Gene Protein 2; MDIG; Mina53; Nucleolar Protein 52; MINA53; NO52) (PE); anti-MINA antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human MINA.
Purity/Purification
Purified by Protein G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-MINA antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:10-1:50
FLISA: 1:1,000
WB: 1:250-1:500
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the C-terminal region of human MINA (KLH).
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-MINA (C-term) Pab pre-incubated with and without blocking peptide (BP)(catlog #:BP1033b) in Jurkat cell line lysate. MINA(C-term)(arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of anti-MINA (C-term) Pab pre-incubated with and without blocking peptide (BP)(catlog #:BP1033b) in Jurkat cell line lysate. MINA(C-term)(arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with MINA (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with MINA (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Western Blot (WB)

(Western blot analysis of MINA (arrow) using rabbit polyclonal MINA Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MINA gene.)

Western Blot (WB) (Western blot analysis of MINA (arrow) using rabbit polyclonal MINA Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MINA gene.)
Product Categories/Family for anti-MINA antibody
References
1. Eilbracht, J., et al., Mol. Biol. Cell 15(4):1816-1832 (2004). 2. Teye, K., et al., Am. J. Pathol. 164(1):205-216 (2004). 3. Tsuneoka, M., et al., J. Biol. Chem. 277(38):35450-35459 (2002).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
53kDa
NCBI Official Full Name
ribosomal oxygenase 2 isoform b
NCBI Official Synonym Full Names
ribosomal oxygenase 2
NCBI Official Symbol
RIOX2
NCBI Official Synonym Symbols
ROX; MDIG; MINA; NO52; JMJD10; MINA53
NCBI Protein Information
ribosomal oxygenase 2
UniProt Protein Name
Bifunctional lysine-specific demethylase and histidyl-hydroxylase MINA
UniProt Gene Name
MINA
UniProt Synonym Gene Names
ROX
UniProt Entry Name
MINA_HUMAN

NCBI Description

MINA is a c-Myc (MYC; MIM 190080) target gene that may play a role in cell proliferation or regulation of cell growth. (Tsuneoka et al., 2002 [PubMed 12091391]; Zhang et al., 2005 [PubMed 15897898]).[supplied by OMIM, May 2008]

Uniprot Description

MINA: Involved in cellular proliferation. May play an important role in cell growth and survival. May be involved in ribosome biogenesis, most likely during the assembly process of pre-ribosomal particles. Belongs to the MINA53/NO66 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus

Chromosomal Location of Human Ortholog: 3q11.2

Cellular Component: transcription factor complex; cytoplasm; nucleolus; cytosol; nucleus

Molecular Function: dioxygenase activity; metal ion binding

Biological Process: transcription, DNA-dependent; ribosome biogenesis and assembly; negative regulation of transcription from RNA polymerase II promoter

Research Articles on MINA

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Product Notes

The MINA mina (Catalog #AAA6488190) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MINA, CT (Protein Mina53, MYC-induced Nuclear Antigen, Mineral Dust-induced Gene Protein 2, MINA, MDIG, Mina53, Nucleolar Protein 52, MINA53, NO52) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MINA can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:10-1:50 FLISA: 1:1,000 WB: 1:250-1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MINA mina for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MINA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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