Rabbit anti-Human CMGA Polyclonal Antibody | anti-CMGA antibody

CMGA, CT (CHGA, SS-18, WA-8, WE-14, LF-19, AL-11, GV-19, GR-44, Chromogranin-A, Pituitary secretory protein I, Vasostatin-1, Vasostatin I, Vasostatin-2, Vasostatin II, EA-92, ES-43, Pancreastatin, ER-37) (Azide free) (HRP)

Cat. Num. AAA6287013

• Gene Names: CHGA; CGA
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: CMGA; Polyclonal Antibody; CT (CHGA; SS-18; WA-8; WE-14; LF-19; AL-11; GV-19; GR-44; Chromogranin-A; Pituitary secretory protein I; Vasostatin-1; Vasostatin I; Vasostatin-2; Vasostatin II; EA-92; ES-43; Pancreastatin; ER-37) (Azide free) (HRP); CHGA; ER-37; anti-CMGA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CMGA.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CMGA antibody: Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 376-404 in the C-terminal region of human CMGA.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in A549 cell line lysates (35ug/lane) usingMBS6002073. This demonstrates that MBS6002073 detected the CMGA protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue using MBS6002073 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6002073 for immunohistochemistry.)

Testing Data

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Testing Data

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Product Categories/Family for anti-CMGA antibody

Antibodies; Chromogranin

References

1. Ezzi, S.A., et al. J. Neurochem. 115(5):1102-1111(2010). 2. Ma, Z., et al. J. Urol. 184(3):1182-1188(2010). 3. Ramella, R., et al. J. Cell. Biochem. 110(1):70-79(2010). 4. Dag, E., et al. Peptides 31(5):932-937(2010). 5. Xie, Y.Q., et al. Zhonghua Xin Xue Guan Bing Za Zhi 37(12):1081-1084(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4502805
• NCBI GeneID: 1113
• NCBI Accession #: NP_001266.1
• NCBI GenBank Nucleotide #: NM_001275.3
• UniProt Accession #: P10645; Q53FA8; Q6NR84; Q96E84; Q96GL7; Q9BQB5; B2R9E9
• Molecular Weight: 50,688 Da
• NCBI Official Full Name: chromogranin-A preproprotein
• NCBI Official Synonym Full Names: chromogranin A (parathyroid secretory protein 1)
• NCBI Official Symbol: CHGA
• NCBI Official Synonym Symbols: CGA
• NCBI Protein Information: chromogranin-A; SP-I; pituitary secretory protein I; parathyroid secretory protein 1; betagranin (N-terminal fragment of chromogranin A)
• UniProt Protein Name: Chromogranin-A
• UniProt Gene Name: CHGA
• UniProt Synonym Gene Names: CgA; SP-I
• UniProt Entry Name: CMGA_HUMAN

NCBI Description

The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Other peptides, including chromostatin, beta-granin, WE-14 and GE-25, are also derived from the full-length protein. However, biological activities for these molecules have not been shown. [provided by RefSeq, Jul 2008]

Uniprot Description

CHGA: a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells and is a precursor to multiple biologically active peptides including vasostatin, pancreastatin, and chromostatin. Binds calcium with a low-affinity. Vasostatin negatively regulates angiogenesis and has antibacterial activity against some Gram-positive bacteria. Pancreastatin inhibits glucose induced insulin release from the pancreas. Chromostatin inhibits catecholamine release from chromaffin cells.

Protein type: Secreted, signal peptide; Vesicle; Cell adhesion; Secreted

Chromosomal Location of Human Ortholog: 14q32

Cellular Component: perinuclear region of cytoplasm; extracellular region; secretory granule

Biological Process: regulation of blood pressure