Rabbit SNAI2 Polyclonal Antibody | anti-SNAI2 antibody

SNAI2 Antibody

Cat. Num. AAA9606861

• Gene Names: SNAI2; SLUG; WS2D; SLUGH; SLUGH1; SNAIL2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: SNAI2; Polyclonal Antibody; SNAI2 Antibody; dJ710H13.1; MGC10182; Neural crest transcription factor Slug; Protein sna; Protein snail homolog 1; Protein snail homolog 2; Protein snail homolog; Slug homolog zinc finger protein; Slug zinc finger protein; SLUGH; SLUGH 1; SLUGH1; SLUGH2; SNA; Sna protein; SNAH; SNAI 2; snai1; SNAI1_HUMAN; Snai2; SNAI2_HUMAN; Snail 2; Snail homolog 1 (Drosophila); Snail homolog 2; Snail2; WS 2D; WS2D; Zinc finger protein SLUG; Zinc finger protein SNAI1; Zinc finger protein SNAI2; anti-SNAI2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SNAI2 antibody detects endogenous levels of total SNAI2
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 268

• Applicable Applications for anti-SNAI2 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: A synthesized peptide derived from human SNAI2
• Subcellular Location: Slug is generally nuclear, while snail is known to be both cytoplasmic and nuclear. Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) snail is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.
• Tissue Specificity: Expressed in most adult human tissues, including spleen, thymus, prostate, testis, ovary, small intestine, colon, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Not detected in peripheral blood leukocyte. Expressed in the dermis and in all layers of the epidermis, with high levels of expression in the basal layers (at protein level). Expressed in osteoblasts (at protein level). Expressed in mesenchymal stem cells (at protein level). Expressed in breast tumor cells (at protein level).
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Immunohistochemistry (IHC)

(SNAI2 Antibody(MBS9606861) staining human ovarian cancer tissue sections by IHC-f at 1:200.(200x).)

Immunohistochemistry (IHC)

(SNAI2 Antibody(MBS9606861) staining human ovarian cancer tissue sections by IHC-f at 1:200.)

Immunohistochemistry (IHC)

(SNAI2 Antibody(MBS9606861) staining human cervical carcinoma tissue sections by IHC-f at 1:200.)

Western Blot (WB)

(Western blot analysis of pancreas lysate using SNAI2 antibody. The lane on the left is treated with the antigen-specific peptide.)

Related Product Information for anti-SNAI2 antibody

Description: Slug (SNAI2) is a widely expressed transcriptional repressor and member of the Snail family of zinc finger transcription factors (1). Similar to the related Snail protein, Slug binds to the E-cadherin promoter region to repress transcription during development (2). The binding of Slug to integrin promoter sequences represses integrin expression and results in reduced cell adhesion (3). Down regulation of E-cadherin expression occurs during the epithelial-mesenchymal transition during embryonic development, a process also exploited by invasive cancer cells (4, 5). The tumor suppressor protein p53 induces Slug expression in gamma-irradiated cells; Slug protects damaged cells from apoptosis by repressing p53-induced transcription of the proapoptotic Bcl-2 family protein Puma (6). Deletion mutations in the corresponding Slug gene are associated with the pigmentation disorders Waardenburg Syndrome and Piebaldism, while a genetic duplication resulting in Slug overexpression is associated with a collection of congenital heart defects termed tetralogy of Fallot (7).
Function: Transcriptional repressor that modulates both activator-dependent and basal transcription. Involved in the generation and migration of neural crest cells. Plays a role in mediating RAF1-induced transcriptional repression of the TJ protein, occludin (OCLN) and subsequent oncogenic transformation of epithelial cells (By similarity). Represses BRCA2 expression by binding to its E2-box-containing silencer and recruiting CTBP1 and HDAC1 in breast cells. In epidermal keratinocytes, binds to the E-box in ITGA3 promoter and represses its transcription. Involved in the regulation of ITGB1 and ITGB4 expression and cell adhesion and proliferation in epidermal keratinocytes. Binds to E-box2 domain of BSG and activates its expression during TGFB1-induced epithelial-mesenchymal transition (EMT) in hepatocytes. Represses E-Cadherin/CDH1 transcription via E-box elements. Involved in osteoblast maturation. Binds to RUNX2 and SOC9 promoters and may act as a positive and negative transcription regulator, respectively, in osteoblasts. Binds to CXCL12 promoter via E-box regions in mesenchymal stem cells and osteoblasts. Plays an essential role in TWIST1-induced EMT and its ability to promote invasion and metastasis.
Subunit Structure: Interacts (via SNAG domain) with LIMD1 (via LIM domains), WTIP (via LIM domains) and AJUBA (via LIM domains) (By similarity). Interacts (via zinc fingers) with KPNA2, KPNB1, and TNPO1. May interact (via zinc fingers) with IPO7.
Post-translational Modifications: GSK3B-mediated phosphorylation results in cytoplasmic localization and degradation.
Similarity: Repression activity depends on the C-terminal DNA-binding zinc fingers and on the N-terminal repression domain. Belongs to the snail C2H2-type zinc-finger protein family.

NCBI and Uniprot Product Information

• NCBI GI #: 11276067
• NCBI GeneID: 6591
• NCBI Accession #: NP_003059.1
• NCBI GenBank Nucleotide #: NM_003068.4
• UniProt Accession #: O43623; Q53FC1; B2R6P6
• Molecular Weight: Observed: 30 kDa; Predicted: 30 kDa
• NCBI Official Full Name: zinc finger protein SNAI2
• NCBI Official Synonym Full Names: snail family transcriptional repressor 2
• NCBI Official Symbol: SNAI2
• NCBI Official Synonym Symbols: SLUG; WS2D; SLUGH; SLUGH1; SNAIL2
• NCBI Protein Information: zinc finger protein SNAI2
• UniProt Protein Name: Zinc finger protein SNAI2
• Protein Family: Zinc finger protein
• UniProt Gene Name: SNAI2
• UniProt Synonym Gene Names: SLUG; SLUGH

NCBI Description

This gene encodes a member of the Snail family of C2H2-type zinc finger transcription factors. The encoded protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in this gene may be associated with sporatic cases of neural tube defects. [provided by RefSeq, Jul 2008]

Uniprot Description

Transcriptional repressor that modulates both activator-dependent and basal transcription. Involved in the generation and migration of neural crest cells. Plays a role in mediating RAF1-induced transcriptional repression of the TJ protein, occludin (OCLN) and subsequent oncogenic transformation of epithelial cells (). Represses BRCA2 expression by binding to its E2-box-containing silencer and recruiting CTBP1 and HDAC1 in breast cells. In epidermal keratinocytes, binds to the E-box in ITGA3 promoter and represses its transcription. Involved in the regulation of ITGB1 and ITGB4 expression and cell adhesion and proliferation in epidermal keratinocytes. Binds to E-box2 domain of BSG and activates its expression during TGFB1-induced epithelial-mesenchymal transition (EMT) in hepatocytes. Represses E-Cadherin/CDH1 transcription via E-box elements. Involved in osteoblast maturation. Binds to RUNX2 and SOC9 promoters and may act as a positive and negative transcription regulator, respectively, in osteoblasts. Binds to CXCL12 promoter via E-box regions in mesenchymal stem cells and osteoblasts. Plays an essential role in TWIST1-induced EMT and its ability to promote invasion and metastasis.