Western Blot (WB)
(Western Blot analysis of MIDN expression in transfected 293T cell line by MIDN polyclonal antibody. Lane 1: MIDN transfected lysate (51.48kD). Lane 2: Non-transfected lysate.)
Mouse anti-Human MIDN Polyclonal Antibody | anti-MIDN antibody
MIDN (Midnolin, Midbrain Nucleolar Protein, DKFZp547M072)
Reactivity
Human
Applications
Western Blot
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
MIDN; Polyclonal Antibody; MIDN (Midnolin; Midbrain Nucleolar Protein; DKFZp547M072); Anti -MIDN (Midnolin; anti-MIDN antibody
Host
Mouse
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human MIDN.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Sequence
MEPQPGGARSCRRGAPGGACELGPAAEAAPMSLAIHSTTGTRYDLAVPPDETVEGLRKRLSQRLKVPKERLALLHKDTRLSSGKLQEFGVGDGSKLTLVPTVEAGLMSQASRPEQSVMQALESLTETQVSDFLSGRSPLTLALRVGDHMMFVQLQLAAQHAPLQHRHVLAAAAAAAAARGDPSIASPVSSPCRPVSSAARVPPVPTSPSPASPSPITAGSFRSHAASTTCPEQMDCSPTASSSASPGASTTSTPGASPAPRSRKPGAVIESFVNHAPGVFSGTFSGTLHPNCQDSSGRPRRDIGTILQILNDLLSATRHYQGMPPSLAQLRCHAQCSPASPAPDLAPRTTSCEKLTAAPSASLLQGQSQIRMCKPPGDRLRQTENRATRCKVERLQLLLQQKRLRRKARRDARGPYHWSPSRKAGRSDSSSSGGGGSPSEASGLGLDFEDSVWKPEANPDIKSEFVVA
Applicable Applications for anti-MIDN antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Immunogen
Full length human MIDN, aa1-468 (AAH94778.1).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of MIDN expression in transfected 293T cell line by MIDN polyclonal antibody. Lane 1: MIDN transfected lysate (51.48kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of MIDN expression in transfected 293T cell line by MIDN polyclonal antibody. Lane 1: MIDN transfected lysate (51.48kD). Lane 2: Non-transfected lysate.)
Related Product Information for anti-MIDN antibody
MIDN may be involved in regulation of genes related to neurogenesis in the nucleolus (By similarity).
Product Categories/Family for anti-MIDN antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
49,213 Da
NCBI Official Full Name
MIDN protein, partial
NCBI Official Synonym Full Names
midnolin
NCBI Official Symbol
MIDN
NCBI Protein Information
midnolin; midbrain nucleolar protein
UniProt Protein Name
Midnolin
Protein Family
UniProt Gene Name
MIDN
UniProt Entry Name
MIDN_HUMAN
Uniprot Description
MIDN: May be involved in regulation of genes related to neurogenesis in the nucleolus.
Protein type: Nucleolus
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: nucleolus; cytosol
Molecular Function: kinase binding
Biological Process: negative regulation of glucokinase activity; negative regulation of insulin secretion
Similar Products
Product Notes
The MIDN midn (Catalog #AAA644998) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MIDN (Midnolin, Midbrain Nucleolar Protein, DKFZp547M072) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MIDN can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Researchers should empirically determine the suitability of the MIDN midn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MEPQPGGARS CRRGAPGGAC ELGPAAEAAP MSLAIHSTTG TRYDLAVPPD ETVEGLRKRL SQRLKVPKER LALLHKDTRL SSGKLQEFGV GDGSKLTLVP TVEAGLMSQA SRPEQSVMQA LESLTETQVS DFLSGRSPLT LALRVGDHMM FVQLQLAAQH APLQHRHVLA AAAAAAAARG DPSIASPVSS PCRPVSSAAR VPPVPTSPSP ASPSPITAGS FRSHAASTTC PEQMDCSPTA SSSASPGAST TSTPGASPAP RSRKPGAVIE SFVNHAPGVF SGTFSGTLHP NCQDSSGRPR RDIGTILQIL NDLLSATRHY QGMPPSLAQL RCHAQCSPAS PAPDLAPRTT SCEKLTAAPS ASLLQGQSQI RMCKPPGDRL RQTENRATRC KVERLQLLLQ QKRLRRKARR DARGPYHWSP SRKAGRSDSS SSGGGGSPSE ASGLGLDFED SVWKPEANPD IKSEFVVA. It is sometimes possible for the material contained within the vial of "MIDN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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