Mouse anti-Human Deleted in Azoospermia-associated Protein 1 Monoclonal Antibody | anti-DAZAP1 antibody

Deleted in Azoospermia-associated Protein 1 (DAZ-associated Protein 1, DAZAP1)

Cat. Num. AAA607626

• Gene Names: DAZAP1; MGC19907
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Deleted in Azoospermia-associated Protein 1; Monoclonal Antibody; Deleted in Azoospermia-associated Protein 1 (DAZ-associated Protein 1; DAZAP1); Anti -Deleted in Azoospermia-associated Protein 1 (DAZ-associated Protein 1; anti-DAZAP1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 9j41
• Specificity: Recognizes human DAZAP1.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 1% BSA and 0.05% sodium azide.

• Applicable Applications for anti-DAZAP1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.
• Immunogen: Synthetic peptide corresponding to the C-terminal region of human DAZAP1
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

Product Categories/Family for anti-DAZAP1 antibody

Antibodies; Abs to Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 25470890
• NCBI GeneID: 26528
• NCBI Accession #: NP_733829.1
• NCBI GenBank Nucleotide #: NM_170711.1
• UniProt Accession #: Q6P657; Q5IRM7; Q96EP5
• Molecular Weight: 6,184 Da
• NCBI Official Full Name: DAZ-associated protein 1 isoform a
• NCBI Official Synonym Full Names: DAZ associated protein 1
• NCBI Official Symbol: DAZAP1
• NCBI Official Synonym Symbols: MGC19907
• NCBI Protein Information: DAZ-associated protein 1; deleted in azoospermia associated protein 1; deleted in azoospermia-associated protein 1
• UniProt Protein Name: PLXND1 protein
• Protein Family: DAZ-associated protein
• UniProt Gene Name: PLXND1
• UniProt Entry Name: Q6P657_HUMAN

NCBI Description

In mammals, the Y chromosome directs the development of the testes and plays an important role in spermatogenesis. A high percentage of infertile men have deletions that map to regions of the Y chromosome. The DAZ (deleted in azoospermia) gene cluster maps to the AZFc region of the Y chromosome and is deleted in many azoospermic and severely oligospermic men. It is thought that the DAZ gene cluster arose from the transposition, amplification, and pruning of the ancestral autosomal gene DAZL also involved in germ cell development and gametogenesis. This gene encodes a RNA-binding protein with two RNP motifs that was originally identified by its interaction with the infertility factors DAZ and DAZL. Two isoforms are encoded by transcript variants of this gene. [provided by RefSeq]

Uniprot Description

DAZAP1: a ubiquitous RNA-binding protein highly expressed in the human and the mouse testes. In round spermatids, it localizes mainly in the nuclei, whereas in elongated spermatids, it localizes to the cytoplasm. Expressed at lower level in thymus and weakly expressed in heart, liver, brain, placenta, lung, skeletal muscle, kidney and pancreas. Fusion proteins between DAZAP1 and MEF2D have been observed in the TS-2 acute lymphoblastic leukemia (ALL) cell line. Two alternatively spliced isoforms have been described.

Protein type: RNA-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: RNA binding; nucleotide binding

Biological Process: positive regulation of nuclear mRNA splicing, via spliceosome; cell proliferation; spermatogenesis; cell differentiation; maternal placenta development