Immunoprecipitation (IP)
(MID1IP1 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate2 uL anti-MID1IP1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-MID1IP1 rabbit polyclonal antibody,at 1:250 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 20 kDaObserved band size: 20 kDa)
Rabbit anti-Human MID1IP1 Polyclonal Antibody | anti-MID1IP1 antibody
Anti-MID1IP1 Antibody, Rabbit Polyclonal
Gene Names
MID1IP1; S14R; MIG12; THRSPL; G12-like; STRAIT11499
Reactivity
Human
Applications
Western Blot, ELISA, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
MID1IP1; Polyclonal Antibody; Anti-MID1IP1 Antibody; Rabbit Polyclonal; MID1IP1 Antibody; Rabbit PAb; Antigen Affinity Purified; MID1 interacting protein 1; Anti-G12-like Antibody; Anti-MIG12 Antibody; Anti-S14R Antibody; Anti-STRAIT11499 Antibody; Anti-THRSPL Antibody; anti-MID1IP1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human MID1IP1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-MID1IP1 antibody
Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
ELISA: 1:5000-1:10,000
IP: 1-4uL/mg of lysate
ELISA: 1:5000-1:10,000
IP: 1-4uL/mg of lysate
Immunogen
Recombinant Human MID1IP1 protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human MID1IP1 (rh MID1IP1). MID1IP1 specific IgG was purified by Human MID1IP1 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunoprecipitation (IP)
(MID1IP1 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate2 uL anti-MID1IP1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-MID1IP1 rabbit polyclonal antibody,at 1:250 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 20 kDaObserved band size: 20 kDa)
Immunoprecipitation (IP)
(MID1IP1 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate2 uL anti-MID1IP1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-MID1IP1 rabbit polyclonal antibody,at 1:250 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 20 kDaObserved band size: 20 kDa)
Western Blot (WB)
(Anti-MID1IP1 rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLane B: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:20 kDaObserved band size:24 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-MID1IP1 rabbit polyclonal antibody at 1:500 dilutionLane A: 293T Whole Cell LysateLane B: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:20 kDaObserved band size:24 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-MID1IP1 antibody
MID1IP1 (MID1 Interacting Protein 1) is a Protein Coding gene. The encoded protein belongs to the SPOT14 family. It is a homodimer in the absence of THRSP. MID1IP1 interacts with ACACA and ACACB. Its interaction with THRSP interferes with ACACA binding. It up-regulates ACACA enzyme activity and plays a role in the regulation of lipogenesis in the liver. MID1IP1 is required for efficient lipid biosynthesis, including triacylglycerol, diacylglycerol, and phospholipid. MID1IP1 is involved in the stabilization of microtubules. It is widely expressed in bone marrow, fat, and other tissues. Diseases associated with MID1IP1 include Gluten Allergy and Scoliosis. Among its related pathways are the Import of palmitoyl-CoA into the mitochondrial matrix and Metabolism.
References
Aipoalani DL. et al., 2010, Endocrinology. 151 (5): 2071-7. Colbert CL. et al., 2010, Proc Natl Acad Sci. 107 (44): 18820-5. Inoue J. et al., 2011, Mol Endocrinol. 25 (6): 995-1005.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,202 Da
NCBI Official Full Name
mid1-interacting protein 1
NCBI Official Synonym Full Names
MID1 interacting protein 1
NCBI Official Symbol
MID1IP1
NCBI Official Synonym Symbols
S14R; MIG12; THRSPL; G12-like; STRAIT11499
NCBI Protein Information
mid1-interacting protein 1; MID1 interacting G12-like protein; MID1 interacting protein 1 (gastrulation specific G12-like); gastrulation specific G12 homolog; spot 14-R; spot 14-related protein
UniProt Protein Name
Mid1-interacting protein 1
Protein Family
UniProt Gene Name
MID1IP1
UniProt Synonym Gene Names
MIG12; S14R; Spot 14-R
UniProt Entry Name
M1IP1_HUMAN
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Product Notes
The MID1IP1 mid1ip1 (Catalog #AAA8107210) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MID1IP1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MID1IP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000 IP: 1-4uL/mg of lysate. Researchers should empirically determine the suitability of the MID1IP1 mid1ip1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MID1IP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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