Kallikrein 6 (KLK6) Recombinant Protein | KLK6 recombinant protein

Recombinant Kallikrein 6 (KLK6)

Cat. Num. AAA2009371

• Gene Names: KLK6; hK6; Bssp; Klk7; SP59; PRSS9; PRSS18
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Kallikrein 6 (KLK6); Recombinant Kallikrein 6 (KLK6); KLK6 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-EEQN KLVHGGPCDK TSHPYQAALY TSGHLLCGGV LIHPLWVLTA AHCKKPNLQV FLGKHNLRQR ESSQEQSSVV RAVIHPDYDA ASHDQDIMLL RLARPAKLSELIQPLPLERD CSANTTSCHI LGWGKTADGD FPDTIQCAYI HLVSREECEH AYPGQITQNM LCAGDEKYGK DSCQGDSGGP LVCGDHLRGLVSWGNIPCGS KEKPGVYTNV CRYTNWIQKT IQAK
• Sequence Length: 244

• Applicable Applications for KLK6 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Glu17~Lys244 (Accession # Q92876) with two N-terminal Tags, His-tag and GST-tag
• Subcellular Location: Secreted. Nucleus, nucleolus. Cytoplasm. Mitochondrion. Microsome
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 6.3
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 61744424
• NCBI GeneID: 5653
• NCBI Accession #: NP_001012982.1
• NCBI GenBank Nucleotide #: NM_001012964.1
• UniProt Accession #: Q92876; Q6H301; A6NJA1; A8MW09
• Molecular Weight: 57.1kDa
• NCBI Official Full Name: kallikrein-6 isoform A preproprotein
• NCBI Official Synonym Full Names: kallikrein-related peptidase 6
• NCBI Official Symbol: KLK6
• NCBI Official Synonym Symbols: hK6; Bssp; Klk7; SP59; PRSS9; PRSS18
• NCBI Protein Information: kallikrein-6; zyme; neurosin; protease M; serine protease 9; serine protease 18
• UniProt Protein Name: Kallikrein-6
• Protein Family: Kallikrein
• UniProt Gene Name: KLK6
• UniProt Synonym Gene Names: PRSS18; PRSS9
• UniProt Entry Name: KLK6_HUMAN

NCBI Description

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. The encoded enzyme is regulated by steroid hormones. In tissue culture, the enzyme has been found to generate amyloidogenic fragments from the amyloid precursor protein, suggesting a potential for involvement in Alzheimer's disease. Multiple alternatively spliced transcript variants that encode different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

KLK6: Serine protease which exhibits a preference for Arg over Lys in the substrate P1 position and for Ser or Pro in the P2 position. Shows activity against amyloid precursor protein, myelin basic protein, gelatin, casein and extracellular matrix proteins such as fibronectin, laminin, vitronectin and collagen. Degrades alpha-synuclein and prevents its polymerization, indicating that it may be involved in the pathogenesis of Parkinson disease and other synucleinopathies. May be involved in regulation of axon outgrowth following spinal cord injury. Tumor cells treated with a neutralizing KLK6 antibody migrate less than control cells, suggesting a role in invasion and metastasis. Belongs to the peptidase S1 family. Kallikrein subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Nucleolus; Secreted; Protease; EC 3.4.21.-

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: nucleoplasm; microtubule cytoskeleton; extracellular space; intercellular bridge; nuclear membrane; mitochondrion; endoplasmic reticulum; cytoplasm; extracellular region; nucleolus

Molecular Function: protein binding; serine-type endopeptidase activity

Biological Process: myelination; protein autoprocessing; collagen catabolic process; central nervous system development; tissue regeneration; amyloid precursor protein metabolic process; positive regulation of G-protein coupled receptor protein signaling pathway; regulation of cell differentiation; response to wounding; hormone metabolic process; protein processing; proteolysis