Rabbit anti-Mouse, Rat MGMT Polyclonal Antibody | anti-MGMT antibody

MGMT Polyclonal Antibody

Cat. Num. AAA2556280

• Gene Names: Mgmt; AGT; Agat; AI267024
• Reactivity: Mouse, Rat
• Applications: Immunohistochemistry
• Purity: Affinity Purification
• Synonyms: MGMT; Polyclonal Antibody; MGMT Polyclonal Antibody; 6 O methylguanine DNA methyltransferase; 6-O-methylguanine-DNA methyltransferase; Agat; AGT; AI267024; EC 2.1.1.63; Methylated DNA protein cysteine methyltransferase; Methylated-DNA--protein-cysteine methyltransferase; Methylguanine DNA methyltransferase; MGC107020; O 6 methylguanine DNA alkyltransferase; O 6 methylguanine DNA methyltransferase; O-6-methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase; anti-MGMT antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Form/Format: PBS with 0.02% sodium azide, 100 mug/ml BSA and 50% glycerol.
• Concentration: 260 mug/mL (varies by lot)
• Sequence Length: 211

• Applicable Applications for anti-MGMT antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:100-1:500
• Immunogen: Recombinant protein corresponding to Mouse MGMT
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded mouse skin using MGMT Polyclonal Antibody at dilution of 1:300.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded mouse uters using MGMT Polyclonal Antibody at dilution of 1:300.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded rat stomach node using MGMT Polyclonal Antibody at dilution of 1:300.)

Related Product Information for anti-MGMT antibody

MGMT (O6-methylguanine-DNA methyltransferase) is transcriptionally activated in response to DNA damage and functions to repair mutagenic and cytotoxic O6-alkylguanine lesions caused by carcinogens or cytostatic drugs. MGMT induction by ionising radiation does not occur in p53-deficient mice, suggesting that MGMT induction may require p53. Similarly, MGMT mRNA and protein were shown to be inducible by ionising radiation, only in cell lines that express functional p53, and not in cell lines that do not express wild type p53. In contrast, high MGMT activity was associated with the presence of mutant p53, in a study of oral cancer cell lines. Similarly, MGMT activity was significantly lower in ovarian tumors with wildtype p53 than in tumors with mutant p53, supporting the view that wildtype p53 down-regulates the basal MGMT promoter.

Product Categories/Family for anti-MGMT antibody

Cancer; Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 6678878
• NCBI GeneID: 17314
• NCBI Accession #: NP_032624.1
• NCBI GenBank Nucleotide #: NM_008598.2
• UniProt Accession #: P26187; Q54A49
• Molecular Weight: 22,435 Da
• NCBI Official Full Name: methylated-DNA--protein-cysteine methyltransferase
• NCBI Official Synonym Full Names: O-6-methylguanine-DNA methyltransferase
• NCBI Official Symbol: Mgmt
• NCBI Official Synonym Symbols: AGT; Agat; AI267024
• NCBI Protein Information: methylated-DNA--protein-cysteine methyltransferase
• UniProt Protein Name: Methylated-DNA--protein-cysteine methyltransferase
• Protein Family: Probable methylated-DNA--protein-cysteine methyltransferase
• UniProt Gene Name: Mgmt
• UniProt Synonym Gene Names: MGMT

Uniprot Description

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.