Western Blot (WB)
(Western Blot analysis of MGMT on different cell lysates using anti- MGMT antibody at 1/1000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: Daudi Lane 4: Raji Lane 5: SK-BR-3 Lane 6: SKOV3 Lane 7: Human liver Lane 8: Human kidney Lane 9: Human thymus Lane 10: Human placentae)
Rabbit anti-Human MGMT Polyclonal Antibody | anti-MGMT antibody
MGMT Polyclonal Antibody
ICC: 1:200
IHC: 1:200
FC/FACS: 1:100
Aliquot store at -20 degree C or -80 degree C.
Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of MGMT on different cell lysates using anti- MGMT antibody at 1/1000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: Daudi Lane 4: Raji Lane 5: SK-BR-3 Lane 6: SKOV3 Lane 7: Human liver Lane 8: Human kidney Lane 9: Human thymus Lane 10: Human placentae)
Western Blot (WB)
(Western Blot analysis of MGMT on different cell lysates using anti- MGMT antibody at 1/1000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: Daudi Lane 4: Raji Lane 5: SK-BR-3 Lane 6: SKOV3 Lane 7: Human liver Lane 8: Human kidney Lane 9: Human thymus Lane 10: Human placentae)
Immunocytochemistry (ICC)
(ICC staining MGMT in A172 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Immunocytochemistry (ICC)
(ICC staining MGMT in A172 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
NCBI and Uniprot Product Information
NCBI Description
Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. The protein encoded by this gene is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma. [provided by RefSeq, Sep 2015]
Uniprot Description
MGMT: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Belongs to the MGMT family.
Protein type: EC 2.1.1.63; Methyltransferase; Nuclear receptor co-regulator; DNA repair, damage; Apoptosis
Chromosomal Location of Human Ortholog: 10q26
Cellular Component: nucleoplasm; membrane; nucleus
Molecular Function: methyltransferase activity; DNA binding; methylated-DNA-[protein]-cysteine S-methyltransferase activity; damaged DNA binding; calcium ion binding; DNA-methyltransferase activity
Biological Process: response to drug; response to ethanol; positive regulation of DNA repair; DNA dealkylation; response to folic acid; response to toxin; DNA methylation; regulation of caspase activity; DNA repair; DNA ligation
Research Articles on MGMT
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Product Notes
The MGMT mgmt (Catalog #AAA3013037) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MGMT Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MGMT can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500-1:1,000 ICC: 1:200 IHC: 1:200 FC/FACS: 1:100. Researchers should empirically determine the suitability of the MGMT mgmt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MGMT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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