RAN recombinant protein

RAN, 1-216aa, Human, His tag, E Coli

Cat. Num. AAA203665

• Gene Names: RAN; TC4; Gsp1; ARA24
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: RAN; 1-216aa; Human; His tag; E Coli; RAN member RAS oncogene family; ARA24; Gsp1; TC4; member RAS oncogene family; Androgen receptor associated protein 24; ARA 24; GTP binding nuclear protein RAN; GTPase Ran; LPS; Ras like protein TC4; RASL2 8; ; RAN recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 10% glycerol
• Concentration: 1mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MAAQGEPQVQ FKLVLVGDGG TGKTTFVKRH LTGEFEKKYV ATLGVEVHPL VFHTNRGPIK FNVWDTAGQE KFGGLRDGYY IQAQCAIIMF DVTSRVTYKN VPNWHRDLVR VCENIPIVLC GNKVDIKDRK VKAKSIVFHR KKNLQYYDIS AKSNYNFEKP FLWLARKLIG DPNLEFVAMP ALAPPEVVMD PALAAQYEHD LEVAQTTALP DEDDDL
• Sequence Length: 216

• Applicable Applications for RAN recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for RAN recombinant protein

RAN, member RAS oncogene family, also known as RAN, is a small GTPase of the Ras superfamily that controls nucleocytoplasmic transport. It is involved in the directionality of the process that is regulated by GTP hydrolysis. Ran shuttles between the nucleus and the cytoplasm. unlike many other regulatory GTPases, Ran is not posttranslationally modified so that it can be targeted to the subcellular compartment where it acts. Rather, RanGTP has been thought to be localized by the differential localization of Ran regulators. Recombinant human RAN, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques

Product Categories/Family for RAN recombinant protein

Signal Transduction

References

Woo IS., et al. (2008) Apoptosis. 13 (10):1223-31.; Ren M., et al. (1994) Mol Cell biol. 14 (6):4216-24.

NCBI and Uniprot Product Information

• NCBI GI #: 5453555
• NCBI GeneID: 5901
• NCBI Accession #: NP_006316
• NCBI GenBank Nucleotide #: NM_006325.4
• UniProt Accession #: P62826; P17080; P28746; P28747; Q6IPB2; Q86V08; Q8NI90; Q9CSP3; Q9CWI7; Q9CZA2; Q9UDJ5; A8K3Z8
• Molecular Weight: 26.5 kDa (236aa), confirmed by MALDI-TOF
• NCBI Official Full Name: GTP-binding nuclear protein Ran isoform 1
• NCBI Official Synonym Full Names: RAN, member RAS oncogene family
• NCBI Official Symbol: RAN
• NCBI Official Synonym Symbols: TC4; Gsp1; ARA24
• NCBI Protein Information: GTP-binding nuclear protein Ran
• UniProt Protein Name: GTP-binding nuclear protein Ran
• Protein Family: Ranakinin
• UniProt Gene Name: RAN
• UniProt Synonym Gene Names: ARA24

NCBI Description

RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis. Because of its many functions, it is likely that RAN interacts with several other proteins. RAN regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. RAN could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. RAN is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). RAN coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease. [provided by RefSeq, Jul 2008]

Uniprot Description

GTPase involved in nucleocytoplasmic transport, participating both to the import and the export from the nucleus of proteins and RNAs. Switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis. Nuclear import receptors such as importin beta bind their substrates only in the absence of GTP-bound RAN and release them upon direct interaction with GTP-bound RAN while export receptors behave in the opposite way. Thereby, RAN controls cargo loading and release by transport receptors in the proper compartment and ensure the directionality of the transport.