Rabbit anti-Human, mouse MGAT3 Polyclonal Antibody | anti-MGAT3 antibody

MGAT3 Antibody (N-term)

Cat. Num. AAA9202964

• Gene Names: MGAT3; GNT3; GNT-III
• Reactivity: Human, mouse
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: MGAT3; Polyclonal Antibody; MGAT3 Antibody (N-term); Beta-1; 4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase; N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase III; GNT-III; GlcNAc-T III; N-acetylglucosaminyltransferase III; GGNT3; anti-MGAT3 antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This MGAT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 72-102 amino acids from the N-terminal region of human MGAT3.
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Positions: 72-102
• Sequence Length: 533

• Applicable Applications for anti-MGAT3 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of MGAT3 antibody (N-term) in mouse spleen tissue lysates (35ug/lane). MGAT3 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-MGAT3 antibody

There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. MGAT3 (N-acetylglucosaminyltransferase III) transfers a GlcNAc residue to the beta-linked mannose of the trimannosyl core of N-linked oligosaccharides and produces a bisecting GlcNAc. Expression of this gene may be controlled by a multiple-promoter system.

References

Shibukawa, Y., et al., J. Biol. Chem. 278(5):3197-3203 (2003).
Koyama, N., et al., Eur. J. Biochem. 238(3):853-861 (1996).
Kim, Y.J., et al., Gene 170(2):281-283 (1996).
Ihara, Y., et al., J. Biochem. 113(6):692-698 (1993).

NCBI and Uniprot Product Information

• NCBI GI #: 148539890
• NCBI GeneID: 4248
• NCBI Accession #: NP_001091740.1; NP_002400.3
• NCBI GenBank Nucleotide #: NM_001098270.1
• UniProt Accession #: Q09327; Q14CK5; Q6IC49; Q9UH32; A6NGD0
• Molecular Weight: 61313
• NCBI Official Full Name: beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
• NCBI Official Synonym Full Names: mannosyl (beta-1,4-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase
• NCBI Official Symbol: MGAT3
• NCBI Official Synonym Symbols: GNT3; GNT-III
• NCBI Protein Information: beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
• UniProt Protein Name: Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
• Protein Family: Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase
• UniProt Gene Name: MGAT3
• UniProt Synonym Gene Names: GGNT3; GNT-III; GlcNAc-T III; N-acetylglucosaminyltransferase III
• UniProt Entry Name: MGAT3_HUMAN

NCBI Description

There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. The enzyme encoded by this gene transfers a GlcNAc residue to the beta-linked mannose of the trimannosyl core of N-linked oligosaccharides and produces a bisecting GlcNAc. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

MGAT3: It is involved in the regulation of the biosynthesis and biological function of glycoprotein oligosaccharides. Catalyzes the addition of N-acetylglucosamine in beta 1-4 linkage to the beta-linked mannose of the trimannosyl core of N-linked sugar chains. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides. Belongs to the glycosyltransferase 17 family.

Protein type: EC 2.4.1.144; Glycan Metabolism - N-glycan biosynthesis; Transferase; Membrane protein, integral

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: Golgi membrane; integral to membrane

Molecular Function: beta-1,4-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity

Biological Process: cellular protein metabolic process; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification