AKR1A1 recombinant protein

Recombinant Human AKR1A1 Protein (His tag)

Cat. Num. AAA2545730

• Gene Names: AKR1A1; ALR; ARM; DD3; ALDR1; HEL-S-6
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: AKR1A1; Recombinant Human AKR1A1 Protein (His tag); RP4-697E16.2; ALDR1; ALR; ARM; DD3; MGC12529; MGC1380; AKR1A1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.5
• Sequence Length: 325

• Application Notes: The recombinant human AKR1A1 consisting of 341 amino acids and migrates as an approximately 39 kDa band in SDS-PAGE under reducing conditions as predicted.
• Predicted N Terminal: Met
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for AKR1A1 recombinant protein

Background: Aldehyde reductase (AKR1A1) is a member of the aldo-keto reductase superfamily, which consists of more than 40 known enzymes and proteins that includes variety of monomeric NADPH-dependent oxidoreductases, such as aldehyde reductase. Aldehyde reductase has wide substrate specificities for carbonyl compounds. These enzymes are implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Aldehyde reductase possess a structure with a beta-alpha-beta fold which contains a novel NADP-binding motif. The binding site is located in a large, deep, elliptical pocket in the C-terminal end of the beta sheet, the substrate being bound in an extended conformation. This binding is more similar to FAD- than to NAD (P)-binding oxidoreductases. AKR1A1 is involved in the reduction of biogenic and xenobiotic aldehydes and is present in virtually every tissue.

Description: A DNA sequence encoding the human AKR1A1 (P14550) (Met 1-Tyr 325) was expressed, with a polyhistidine tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 320202988
• NCBI GeneID: 10327
• NCBI Accession #: NP_001189342.1
• NCBI GenBank Nucleotide #: NM_001202413.1
• UniProt Accession #: P14550; Q6IAZ4; A8KAL8; D3DQ04
• Molecular Weight: 36,573 Da
• NCBI Official Full Name: alcohol dehydrogenase
• NCBI Official Synonym Full Names: aldo-keto reductase family 1 member A1
• NCBI Official Symbol: AKR1A1
• NCBI Official Synonym Symbols: ALR; ARM; DD3; ALDR1; HEL-S-6
• NCBI Protein Information: alcohol dehydrogenase [NADP(+)]
• UniProt Protein Name: Alcohol dehydrogenase [NADP(+)]
• Protein Family: Alcohol dehydrogenase
• UniProt Gene Name: AKR1A1
• UniProt Synonym Gene Names: ALDR1; ALR

NCBI Description

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member, also known as aldehyde reductase, is involved in the reduction of biogenic and xenobiotic aldehydes and is present in virtually every tissue. Multiple alternatively spliced transcript variants of this gene exist, all encoding the same protein. [provided by RefSeq, Jan 2011]

Uniprot Description

AKR1A1: Catalyzes the NADPH-dependent reduction of a variety of aromatic and aliphatic aldehydes to their corresponding alcohols. Catalyzes the reduction of mevaldate to mevalonic acid and of glyceraldehyde to glycerol. Has broad substrate specificity. In vitro substrates include succinic semialdehyde, 4- nitrobenzaldehyde, 1,2-naphthoquinone, methylglyoxal, and D- glucuronic acid. Plays a role in the activation of procarcinogens, such as polycyclic aromatic hydrocarbon trans-dihydrodiols, and in the metabolism of various xenobiotics and drugs, including the anthracyclines doxorubicin (DOX) and daunorubicin (DAUN). Belongs to the aldo/keto reductase family.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 1.1.1.2; Lipid Metabolism - glycerolipid; Oxidoreductase

Chromosomal Location of Human Ortholog: 1p34.1

Cellular Component: cytosol; extracellular space

Molecular Function: aldo-keto reductase activity; electron carrier activity; L-glucuronate reductase activity; protein binding

Biological Process: aldehyde metabolic process; glucose metabolic process; glucuronate catabolic process to xylulose 5-phosphate