Immunohistochemistry (IHC)
(Immunochemical staining of human METTL21B in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Rabbit anti-Human METTL21B Polyclonal Antibody | anti-EEF1AKMT3 antibody
Anti-METTL21B Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
Protein A & Antigen Affinity
Synonyms
METTL21B; Polyclonal Antibody; Anti-METTL21B Antibody; Rabbit Polyclonal; METTL21B/EEF1AKMT3 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-FAM119B Antibody; anti-EEF1AKMT3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human METTL21B/EEF1AKMT3
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-EEF1AKMT3 antibody
Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
IHC-P: 1:50-1:200
ICC/IF: 1:100-1:500
ICC/IF: 1:100-1:500
Immunogen
E Coli-derived Human METTL21B/EEF1AKMT3 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human METTL21B/EEF1AKMT3 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of human METTL21B in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human METTL21B in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human METTL21B in human lymph node with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Immunohistochemistry (IHC)
(Immunochemical staining of human METTL21B in human lymph node with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)
Immunofluorescence (IF)
(Immunofluorescence staining of METTL21B in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human METTL21B polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)
Immunofluorescence (IF)
(Immunofluorescence staining of METTL21B in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human METTL21B polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)
Related Product Information for anti-EEF1AKMT3 antibody
METTL21B (also known as EEF1AKMT3, FAM119B) is a member of the Family 16 group of methyltransferases. Despite its close relation to the other METTL21 proteins (METTL21A, C and D), METTL21B has a different substrate to both METTL21A (HSPA-KMT) and METTL21D (VCP-KMT). The main difference between these proteins is their N-terminal regions, beyond the core catalytic seven-beta-strand fold, which may therefore be responsible determining their substrate specificities. METTL21B is expressed at medium to high levels in every tissue type, and has been reported to localize to the nucleus, the cytoplasm and centrosomes. METTL21B has been implicated in multiple sclerosis because of changes in its expression in a susceptibility genotype.
References
Hamey JJ, et al. (2017) Mettl21b is a novel human lysine methyltransferase of translation elongation factor 1a: Discovery by crispr/cas9 knockout. Mol Cell Proteomics 16 (12): 2229-2242.
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Product Notes
The EEF1AKMT3 (Catalog #AAA8109917) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-METTL21B Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's METTL21B can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF). IHC-P: 1:50-1:200 ICC/IF: 1:100-1:500. Researchers should empirically determine the suitability of the EEF1AKMT3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "METTL21B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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