Rabbit anti-Human METTL10 Polyclonal Antibody | anti-METTL10 antibody

METTL10, NT (METTL10, C10orf138, Methyltransferase-like protein 10) (APC)

Cat. Num. AAA6320383

• Gene Names: METTL10; C10orf138; Em:AC068896.3
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: METTL10; Polyclonal Antibody; NT (METTL10; C10orf138; Methyltransferase-like protein 10) (APC); Methyltransferase-like protein 10; anti-METTL10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-METTL10 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: METTL10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-65 amino acids from the N-terminal region of human METTL10.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(METTL10 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the METTL10 antibody detected the METTL10 protein (arrow).)

Immunohistochemistry (IHC)

(METTL10 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of METTL10 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(METTL10 Antibody (N-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-METTL10 antibody

Antibodies; Enzymes; Methyltransferase

References

Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 58219056
• NCBI GeneID: 399818
• NCBI Accession #: NP_997719.2
• NCBI GenBank Nucleotide #: NM_212554.2
• UniProt Accession #: Q5JPI9; A8MPY7
• Molecular Weight: 31,830 Da
• NCBI Official Full Name: methyltransferase-like protein 10
• NCBI Official Synonym Full Names: methyltransferase like 10
• NCBI Official Symbol: METTL10
• NCBI Official Synonym Symbols: C10orf138; Em:AC068896.3
• NCBI Protein Information: methyltransferase-like protein 10
• UniProt Protein Name: Methyltransferase-like protein 10
• Protein Family: Protein-lysine N-methyltransferase
• UniProt Gene Name: METTL10
• UniProt Synonym Gene Names: C10orf138
• UniProt Entry Name: MET10_HUMAN

Uniprot Description

METTL10: Belongs to the methyltransferase superfamily.

Protein type: EC 2.1.1.-; Methyltransferase, protein arginine; Methyltransferase

Chromosomal Location of Human Ortholog: 10q26.13

Molecular Function: methyltransferase activity

Biological Process: methylation