Rabbit anti-Human METTL10 Polyclonal Antibody | anti-METTL10 antibody

METTL10 Antibody (N-term)

Cat. Num. AAA9201485

• Gene Names: METTL10; C10orf138
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: METTL10; Polyclonal Antibody; METTL10 Antibody (N-term); Methyltransferase-like protein 10; 211-; C10orf138; anti-METTL10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This METTL10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-57 amino acids from the N-terminal region of human METTL10.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Positions: 29-57
• Sequence Length: 291

• Applicable Applications for anti-METTL10 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000; IHC-P~~1:50~100; FC~~1:10~50
• Function: Protein-lysine methyltransferase that selectively catalyzes the trimethylation of EEF1A at 'Lys-318'.
• Cellular Location: Cytoplasm{ECO:0000255|HAMAP-Rule:MF_03188,ECO:0000269|PubMed:25144183}. Nucleus{ECO:0000255|HAMAP-Rule:MF_03188,ECO:0000269|PubMed:25144183}
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Anti-METTL10 Antibody (N-term) at 1:1000 dilution + HL-60 whole cell lysateLysates/proteins at 20 ?g per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted bandsize : 32 kDa Blocking/Dilution buffer: 5% NFDM/TBST..)

Immunohistochemistry (IHC)

(METTL10 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of METTL10 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(METTL10 Antibody (N-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-METTL10 antibody

Cell Biology

References

Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006)

NCBI and Uniprot Product Information

• NCBI GI #: 58219056
• NCBI GeneID: 399818
• NCBI Accession #: NP_997719.2
• NCBI GenBank Nucleotide #: NM_212554.3
• UniProt Accession #: Q5JPI9; A8MPY7
• Molecular Weight: 31830
• NCBI Official Full Name: protein-lysine N-methyltransferase METTL10 isoform 1
• NCBI Official Synonym Full Names: methyltransferase like 10
• NCBI Official Symbol: METTL10
• NCBI Official Synonym Symbols: C10orf138
• NCBI Protein Information: protein-lysine N-methyltransferase METTL10
• UniProt Protein Name: Protein-lysine N-methyltransferase METTL10
• Protein Family: Protein-lysine N-methyltransferase
• UniProt Gene Name: METTL10
• UniProt Entry Name: MET10_HUMAN

Uniprot Description

METTL10: Belongs to the methyltransferase superfamily.

Protein type: Methyltransferase; EC 2.1.1.-; Methyltransferase, protein arginine

Chromosomal Location of Human Ortholog: 10q26.13

Molecular Function: methyltransferase activity

Biological Process: methylation