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Western Blot (WB) (Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: K562 and 293T cell lysates, Primary antibody: 823713(METAP1D Antibody) at dilution 1/600, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes)

Rabbit anti-Human METAP1D Polyclonal Antibody | anti-METAP1D antibody

METAP1D Rabbit pAb

Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
METAP1D; Polyclonal Antibody; METAP1D Rabbit pAb; MAP 1D; Metap1l; MetAP 1D; anti-METAP1D antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Affinity purified
Form/Format
-20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Applicable Applications for anti-METAP1D antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:2000
Immunogen
Fusion protein of human METAP1D
Conjugation
Unconjugated
Molecular Function
The N-terminal methionine excision pathway is an essential process in which the N-terminal methionine is removed from many proteins, thus facilitating subsequent protein modification. In mitochondria, enzymes that catalyze this reaction are celled methionine aminopeptidases (MetAps, or MAPs; EC 3.4.11.18) (Serero et al., 2003 [PubMed 14532271]).
Modification
Unmodified
Cell Position
Mitochondrion
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: K562 and 293T cell lysates, Primary antibody: 823713(METAP1D Antibody) at dilution 1/600, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes)

Western Blot (WB) (Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: K562 and 293T cell lysates, Primary antibody: 823713(METAP1D Antibody) at dilution 1/600, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes)

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human brain tissue using 823713(METAP1D Antibody) at dilution 1/60. (Original magnification: ×200))

Immunohistochemistry (IHC) (The image is immunohistochemistry of paraffin-embedded Human brain tissue using 823713(METAP1D Antibody) at dilution 1/60. (Original magnification: ×200))

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 823713(METAP1D Antibody) at dilution 1/60. (Original magnification: ×200))

Immunohistochemistry (IHC) (The image is immunohistochemistry of paraffin-embedded Human lung cancer tissue using 823713(METAP1D Antibody) at dilution 1/60. (Original magnification: ×200))
Product Categories/Family for anti-METAP1D antibody

NCBI and Uniprot Product Information

UniProt Accession #
Molecular Weight
37kDa
UniProt Protein Name
Methionine aminopeptidase 1D, mitochondrial
UniProt Gene Name
METAP1D
UniProt Entry Name
MAP12_HUMAN

Uniprot Description

MAP1D: Removes the N-terminal methionine from nascent proteins. May play a role in colon tumorigenesis. Belongs to the peptidase M24A family.

Protein type: EC 3.4.11.18; Protease; Mitochondrial

Chromosomal Location of Human Ortholog: 2q31.1

Cellular Component: mitochondrion

Molecular Function: metalloexopeptidase activity; metal ion binding; aminopeptidase activity

Biological Process: peptidyl-methionine modification; proteolysis; N-terminal protein amino acid modification

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Product Notes

The METAP1D metap1d (Catalog #AAA4758103) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The METAP1D Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's METAP1D can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the METAP1D metap1d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "METAP1D, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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