Rabbit anti-Human Merlin Polyclonal Antibody | anti-NF2 antibody

Merlin, NT (Moesin-ezrin-radixin-like Protein, ACN, BANF, Neurofibromin 2, Neurofibromin-2, NF2, Schwannomerlin, Schwannomin, SCH) (MaxLight 650)

Cat. Num. AAA6487764

• Gene Names: NF2; ACN; SCH; BANF
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: Merlin; Polyclonal Antibody; NT (Moesin-ezrin-radixin-like Protein; ACN; BANF; Neurofibromin 2; Neurofibromin-2; NF2; Schwannomerlin; Schwannomin; SCH) (MaxLight 650); Bilateral Acoustic Neuroma; anti-NF2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Merlin.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.
• Sequence Length: 595

• Applicable Applications for anti-NF2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human Merlin (KLH).
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of Merlin Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane). NF2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Merlin Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Merlin Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of Merlin Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-NF2 antibody

Antibodies; Tumor Suppressors

References

1. James, M.F., et.al., Mol. Cell. Biol. 29(15), 4250-4261 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4557795
• NCBI GeneID: 4771
• NCBI Accession #: NP_000259
• NCBI GenBank Nucleotide #: NP_000259.1
• UniProt Accession #: P35240
• Molecular Weight: 69.69
• NCBI Official Full Name: merlin isoform 1
• NCBI Official Synonym Full Names: neurofibromin 2
• NCBI Official Symbol: NF2
• NCBI Official Synonym Symbols: ACN; SCH; BANF
• NCBI Protein Information: merlin
• UniProt Protein Name: Merlin
• Protein Family: Merlin
• UniProt Gene Name: NF2
• UniProt Synonym Gene Names: SCH
• UniProt Entry Name: MERL_HUMAN

NCBI Description

This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that are thought to link cytoskeletal components with proteins in the cell membrane. This gene product has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. This gene is expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities. Two predominant isoforms and a number of minor isoforms are produced by alternatively spliced transcripts. [provided by RefSeq, Jul 2008]

Uniprot Description

Merlin: a moesin-ezrin-radizin-like protein. Interacts with cell-surface proteins, proteins involved in cytoskeletal dynamics and proteins involved in regulating ion transport. Expressed at high levels during embryonic development; in adults, significant expression is found in Schwann cells, meningeal cells, lens and nerve. Mutations are associated with neurofibromatosis type II. Two predominant isoforms and eight minor isoforms are produced by alternatively spliced transcripts.

Protein type: Cytoskeletal; Tumor suppressor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: filopodium membrane; cortical actin cytoskeleton; early endosome; cytoskeleton; extrinsic to membrane; adherens junction; membrane; perinuclear region of cytoplasm; apical part of cell; lamellipodium; cytoplasm; nucleolus; plasma membrane; nucleus; cleavage furrow

Molecular Function: protein binding; actin binding

Biological Process: intercellular junction assembly and maintenance; negative regulation of MAPKKK cascade; hippocampus development; regulation of protein stability; negative regulation of tyrosine phosphorylation of Stat5 protein; negative regulation of cell-matrix adhesion; odontogenesis of dentine-containing teeth; negative regulation of cell proliferation; Schwann cell proliferation; mesoderm formation; negative regulation of DNA replication; positive regulation of stress fiber formation; ectoderm development; negative regulation of tyrosine phosphorylation of Stat3 protein; negative regulation of protein kinase activity; negative regulation of cell-cell adhesion; actin cytoskeleton organization and biogenesis; positive regulation of cell differentiation; negative regulation of cell migration; negative regulation of JAK-STAT cascade

Disease: Meningioma, Familial, Susceptibility To; Neurofibromatosis, Type Ii; Schwannomatosis 1