Mouse Cytokeratin 8 (KRT8) Monoclonal Antibody | anti-KRT8 antibody

Cytokeratin 8 (KRT8)

Cat. Num. AAA4380064

• Gene Names: KRT8; K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
• Reactivity: Human, Sheep, Rabbit, Cow, Pig.; Does not react with Mouse, Rat, Chicken and Xenopus laevis.
• Applications: Western Blot, Immunohistochemistry
• Synonyms: Cytokeratin 8 (KRT8); Monoclonal Antibody; CARD2; CK8; CYK8; CYKER; Cytokeratin Endo A; DreK8; EndoA; K2C8; K8; Keratin 8; Krt 2.8; KRT8; Type-II Keratin Kb8; anti-KRT8 antibody

• Host: Mouse
• Reactivity: Human, Sheep, Rabbit, Cow, Pig.; Does not react with Mouse, Rat, Chicken and Xenopus laevis.
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: rB22.1
• Specificity: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Reportedly, anti-CK8 is useful for the differentiation of lobular (ring-like, perinuclear) from ductal (peripheral-predominant) carcinoma of the breast.
• Form/Format: 200ug/mL of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide.; Also available WITHOUT BSA and azide at 1.0mg/mL
• Sequence Length: 483

• Applicable Applications for anti-KRT8 antibody: Western Blot (WB), Immunohistochemistry (IHC) Formalin
• Application Notes: Western Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
• Immunogen: Recombinant full-length human KRT8 protein
• Cellular Localization: Cytoplasmic
• Hu-chromosome Location: 12q13.13
• Positive Control: HCT116, MCF-7 or A431 cells, Skin, Colon, Lung or Breast Carcinoma.
• Preparation and Storage: Antibody without azide , store at -20°C to -80°C.; Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Colon stained with Cytokeratin 8 Mouse Recombinant Monoclonal Antibody (rB22.1).)

SDS-Page

(SDS-PAGE Analysis of Purified Cytokeratin 8 Mouse Recombinant Monoclonal Antibody (rB22.1).)

Western Blot (WB)

(Western Blot analysis of HCT116 cell lysate using Cytokeratin 8 Mouse Monoclonal Antibody (rB22.1).)

References

Bartek et al. J. Pathology, 164:215-24 (1991). Kovarik et al. J.Tumor Marker Oncol. 5: 219 (1990). Vojtsek et al. Folia Biol.35: 373-382 (1989).

NCBI and Uniprot Product Information

• NCBI GI #: 372466577
• NCBI GeneID: 3856
• NCBI Accession #: NP_001243222.1
• NCBI GenBank Nucleotide #: NM_001256293.1
• UniProt Accession #: P05787; Q14099; Q14716; Q14717; Q53GJ0; Q6DHW5; Q6GMY0; Q6P4C7; Q96J60; A8K4H3; B0AZN5; F8VXB4
• Molecular Weight: 52.5kDa
• NCBI Official Full Name: keratin, type II cytoskeletal 8 isoform 2
• NCBI Official Synonym Full Names: keratin 8
• NCBI Official Symbol: KRT8
• NCBI Official Synonym Symbols: K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
• NCBI Protein Information: keratin, type II cytoskeletal 8
• UniProt Protein Name: Keratin, type II cytoskeletal 8
• Protein Family: Keratin
• UniProt Gene Name: KRT8
• UniProt Synonym Gene Names: CYK8; CK-8; K8

NCBI Description

This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.