Rabbit VE-Cadherin Polyclonal Antibody | anti-VE antibody

VE-Cadherin Polyclonal Antibody

Cat. Num. AAA3008427

• Gene Names: CDH5; 7B4; CD144
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: VE-Cadherin; Polyclonal Antibody; VE-Cadherin Polyclonal Antibody; CDH5; Cadherin-5; 7B4 antigen; Vascular endothelial cadherin; CD144; anti-VE antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: VE-Cadherin polyclonal antibody detects endogenous levels of VE-Cadherin protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 784

• Applicable Applications for anti-VE antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IF: 1:20-1:100
• Immunogen: Recombinant protein of human CDH5.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of various cell lines, using CDH5 antibody.)

Related Product Information for anti-VE antibody

The cadherins are a family of Ca 2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. Cadherins each contain a large extracellular domain at the amino-terminus, which is characterized by a series of five homologous repeats, the most distal of which is thought to be responsible for binding specificity. The relatively short carboxy-terminal, intracellular domain interacts with a variety of cytoplasmic proteins, including beta-catenin, to regulate cadherin function. VE-cadherin (for vascular endothelial cadherin, also designated cadherin-5) is localized at intercellular junctions of endothelial cells, where it is thought to play a role in the cohesion and organization of intercellular junctions.

NCBI and Uniprot Product Information

• NCBI GI #: 166362713
• NCBI GeneID: 1003
• NCBI Accession #: NP_001786.2
• NCBI GenBank Nucleotide #: NP_001786.2
• UniProt Accession #: P33151
• Molecular Weight: ~ 120kDa
• NCBI Official Full Name: cadherin-5 preproprotein
• NCBI Official Synonym Full Names: cadherin 5
• NCBI Official Symbol: CDH5
• NCBI Official Synonym Symbols: 7B4; CD144
• NCBI Protein Information: cadherin-5
• UniProt Protein Name: Cadherin-5
• UniProt Gene Name: CDH5
• UniProt Synonym Gene Names: VE-cadherin
• UniProt Entry Name: CADH5_HUMAN

NCBI Description

This gene encodes a classical cadherin of the cadherin superfamily. The encoded preproprotein is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion molecule is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classical cadherin by imparting to cells the ability to adhere in a homophilic manner, this protein plays a role in endothelial adherens junction assembly and maintenance. This gene is located in a gene cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. [provided by RefSeq, Nov 2015]

Uniprot Description

CDH5: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for the localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction. Interacts via cadherin 5 domain with PTPRB. Interacts with TRPC4. Interacts with KRIT1. Endothelial tissues and brain.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: adherens junction; tight junction; membrane; plasma membrane; integral to membrane; intercellular junction; cell junction

Molecular Function: protein binding; beta-catenin binding; calcium ion binding; protein phosphatase binding; receptor binding

Biological Process: intercellular junction assembly and maintenance; negative regulation of cell proliferation; homophilic cell adhesion; vascular endothelial growth factor receptor signaling pathway; blood vessel maturation