Testing Data
Rabbit anti-Human Membrane-Type Matrix Metalloproteinase-2 Polyclonal Antibody
Anti-Membrane-Type Matrix Metalloproteinase-2 (MT2-MMP, MMP-15), Hinge Region Antibody
Reactivity
Human
Applications
Western Blot
Purity
Immunogen Affinity Purified
Synonyms
Membrane-Type Matrix Metalloproteinase-2; Polyclonal Antibody; Anti-Membrane-Type Matrix Metalloproteinase-2 (MT2-MMP; MMP-15); Hinge Region Antibody; MMP15; anti-Membrane-Type Matrix Metalloproteinase-2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
The antibody binds to the hinge region of MMP15, and can recognize native or reduced MMP15. It does not cross react with the other MMP family members (MMP1, MMP2, MMP3, MMP9).
Purity/Purification
Immunogen Affinity Purified
Form/Format
Liquid; PBS, pH 7.4 with 0.05% sodium azide.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-Membrane-Type Matrix Metalloproteinase-2 antibody
Western Blot (WB)
Application Notes
MMP-15, first described as MT-MMP2, is a metalloproteinase with type-I transmembrane domain. Initially reported in lung tissue, MMP-15 message was also seen in testis, intestines, colon, heart, liver, kidney, pancreas and muscle. Western blots showed bands at 72 kD, 48 kD and 44 kD. Subsequent publications have focused on message, and found MMP-15 in a wide range of tumor cell lines, as well as the synovial membrane from patients with rheumatoid arthritis. MMP-15 is usually found in much lower levels than the more ubiquitous MMP-14 (MT1MMP). RP1MMP15 works well for Western Blotting applications, and when used against the reduced protein identifies bands at 72 Kd and 55 Kd as well as activation/breakdown product at 48 kD. Often the MMP-15 is fully activated, and the 48 kD band is the dominant band seen. MMP-15 may be shed into the culture media when activated, but it is unclear if the shed form is proteolytically active. Cell lysates are the best source for Western blotting of MMP-15. The antibody can recognize native or reduced MT2MMP, and has application for immunoprecipitation, immunohistochemistry, cell sorting and ELISA. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentration of antibody may be needed for non-human samples.
Immunogen
Synthetic peptide based on a sequence from Human MMP15.
Cellular Localization
Membrane; Single Pass Type I Membrane Protein.
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Testing Data
Testing Data
Related Product Information for anti-Membrane-Type Matrix Metalloproteinase-2 antibody
Description: The antibody is a Rabbit antibody to the membrane-bound Matrix Metalloproteinase-2 (MMP-15), made with a synthetic peptide based on the Human sequence. The antibody binds to the hinge region of MT2MMP. The antibody binds to MT2MMP, but does not cross react with the other MMP family members (MMP1, MMP2, MMP3, MMP-9). The antibody has been peptide-affinity purified, concentrated to 1 mg/ml, with the addition of Sodium Azide as preservative, and 50% glycerol as cryoprotectant.
Relevance: The mammalian matrix metalloproteinases (MMPs) degrade extracellular matrix in physiological and pathological processes. Of the sixteen members of matrix metalloproteinase family ten exist in soluble form whereas MT MMPs exist as integral membrane proteins. MMP15, first described as MTMMP2, is a metalloproteinase with a type I transmembrane domain. Initially reported in lung tissue, MMP15 message was also seen in testis, intestines, colon, heart, liver, kidney, pancreas and muscle. Western blots showed bands at 72 kD, 48 kD and 44 kD. Subsequent publications have focused on message, and found MMP15 in a wide range of tumor cell lines, as well as the synovial membrane from patients with rheumatoid arthritis. MMP15 is usually found in much lower levels than the more ubiquitous MMP14 (MT1MMP). MMP15 may be shed into the culture media when activated, but it is unclear if the shed form is proteolytically active. Cell lysates are the best source for Western blotting of MMP15.
Relevance: The mammalian matrix metalloproteinases (MMPs) degrade extracellular matrix in physiological and pathological processes. Of the sixteen members of matrix metalloproteinase family ten exist in soluble form whereas MT MMPs exist as integral membrane proteins. MMP15, first described as MTMMP2, is a metalloproteinase with a type I transmembrane domain. Initially reported in lung tissue, MMP15 message was also seen in testis, intestines, colon, heart, liver, kidney, pancreas and muscle. Western blots showed bands at 72 kD, 48 kD and 44 kD. Subsequent publications have focused on message, and found MMP15 in a wide range of tumor cell lines, as well as the synovial membrane from patients with rheumatoid arthritis. MMP15 is usually found in much lower levels than the more ubiquitous MMP14 (MT1MMP). MMP15 may be shed into the culture media when activated, but it is unclear if the shed form is proteolytically active. Cell lysates are the best source for Western blotting of MMP15.
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Product Notes
The Membrane-Type Matrix Metalloproteinase-2 (Catalog #AAA4157321) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Membrane-Type Matrix Metalloproteinase-2 (MT2-MMP, MMP-15), Hinge Region Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Membrane-Type Matrix Metalloproteinase-2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). MMP-15, first described as MT-MMP2, is a metalloproteinase with type-I transmembrane domain. Initially reported in lung tissue, MMP-15 message was also seen in testis, intestines, colon, heart, liver, kidney, pancreas and muscle. Western blots showed bands at 72 kD, 48 kD and 44 kD. Subsequent publications have focused on message, and found MMP-15 in a wide range of tumor cell lines, as well as the synovial membrane from patients with rheumatoid arthritis. MMP-15 is usually found in much lower levels than the more ubiquitous MMP-14 (MT1MMP). RP1MMP15 works well for Western Blotting applications, and when used against the reduced protein identifies bands at 72 Kd and 55 Kd as well as activation/breakdown product at 48 kD. Often the MMP-15 is fully activated, and the 48 kD band is the dominant band seen. MMP-15 may be shed into the culture media when activated, but it is unclear if the shed form is proteolytically active. Cell lysates are the best source for Western blotting of MMP-15. The antibody can recognize native or reduced MT2MMP, and has application for immunoprecipitation, immunohistochemistry, cell sorting and ELISA. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentration of antibody may be needed for non-human samples. Researchers should empirically determine the suitability of the Membrane-Type Matrix Metalloproteinase-2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Membrane-Type Matrix Metalloproteinase-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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