Testing Data
Rabbit anti-Human, Mouse Membrane-Type Matrix Metalloproteinase-5 Polyclonal Antibody
Anti-Membrane-Type Matrix Metalloproteinase-5 (MT5-MMP, MMP-24), Propeptide Domain MMP-24 Antibody
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Immunogen Affinity Purified
Synonyms
Membrane-Type Matrix Metalloproteinase-5; Polyclonal Antibody; Anti-Membrane-Type Matrix Metalloproteinase-5 (MT5-MMP; MMP-24); Propeptide Domain MMP-24 Antibody; MMP-24; anti-Membrane-Type Matrix Metalloproteinase-5 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
This antibody binds to the propeptide domain of MMP24. It recognizes only the zymogen of MMP24, and not the activated forms. It can recognize native or reduced forms. The antibody does not cross react with the other MMP family members (MMP1, MMP2, MMP3, MMP9).
Purity/Purification
Immunogen Affinity Purified
Form/Format
Liquid; PBS, pH 7.4 with 0.05% sodium azide.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-Membrane-Type Matrix Metalloproteinase-5 antibody
Western Blot (WB)
Application Notes
The antibody works well for Western Blotting applications, and when used against the reduced protein identifies bands at 65 Kd. The antibody recognizes only the zymogen of MMP-24, and not the activated forms. The antibody recognizes human and murine MMP-24. The antibody can recognize native or reduced MT5MMP, and has application for immunoprecipitation, immunohistochemistry, cell sorting and ELISA. MT5MMP has been reported to be elevated in several tumor cell lines, and is constituitively produced by some normal cell lines. MMP-24 is also found in brain tissue, along with other MT-MMP enzymes. Treatment of cells with Concanavolin-A or the phorbol ester TPA stimulates production of MT5MMP in some cell types, and the enzyme can be recovered in cell lysates. Shed forms of MT5MMP have also been reported. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentration of antibody may be needed for non-human samples.
Immunogen
Synthetic peptide based on the Propeptide domain of Mouse MMP24.
Cellular Localization
Cell Membrane; Single-Pass Type I Membrane Protein. Processed form: Secreted protein. Also shed from cell surface as soluble proteinase, by a proteolytic cleavage.
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Testing Data
Testing Data
Related Product Information for anti-Membrane-Type Matrix Metalloproteinase-5 antibody
Description: The antibody is a Rabbit antibody to the membrane-bound Matrix Metalloproteinase-5 (MMP-24), made with a synthetic peptide based on the mouse sequence. The antibody binds to the Propeptide domain of MT5MMP. The antibody binds to MT5MMP, but does not cross react with the other MMP family members (MMP1, MMP2, MMP3, MMP-9). The antibody has been peptide-affinity purified, concentrated to 1 mg/ml, with the addition of Sodium Azide as preservative, and 50% glycerol as cryoprotectant.
Relevance: Matrix Metalloproteinase 24 (MMP24, MT5MMP) has been described as a proteoglycanase, which is critical in the turnover of ECM (extracellular matrix) components in the brain. MMP24 contains a candidate signal sequence, a propeptide region, a potential furin recognition motif, a zinc-binding catalytic domain, a hemopexin-like domain, a 24-residue hydrophobic region, which acts as a potential transmembrane domain, and a short cytosolic domain. Unlike other MTMMPs, MMP24 (MT5MMP) sheds from the cell surface as soluble proteinases, allowing its versatility as both a cell bound and soluble proteinase in ECM remodeling processes. MMP24 is elevated in several tumor cell lines, and is also produced by some normal cell lines. It is predominantly expressed in the brain, indicating a role in nervous system development. MMP24 is also highly expressed during embryonic development. The mouse MMP24 gene maps to chromosome 2.
Relevance: Matrix Metalloproteinase 24 (MMP24, MT5MMP) has been described as a proteoglycanase, which is critical in the turnover of ECM (extracellular matrix) components in the brain. MMP24 contains a candidate signal sequence, a propeptide region, a potential furin recognition motif, a zinc-binding catalytic domain, a hemopexin-like domain, a 24-residue hydrophobic region, which acts as a potential transmembrane domain, and a short cytosolic domain. Unlike other MTMMPs, MMP24 (MT5MMP) sheds from the cell surface as soluble proteinases, allowing its versatility as both a cell bound and soluble proteinase in ECM remodeling processes. MMP24 is elevated in several tumor cell lines, and is also produced by some normal cell lines. It is predominantly expressed in the brain, indicating a role in nervous system development. MMP24 is also highly expressed during embryonic development. The mouse MMP24 gene maps to chromosome 2.
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Product Notes
The Membrane-Type Matrix Metalloproteinase-5 (Catalog #AAA4157352) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Membrane-Type Matrix Metalloproteinase-5 (MT5-MMP, MMP-24), Propeptide Domain MMP-24 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Membrane-Type Matrix Metalloproteinase-5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). The antibody works well for Western Blotting applications, and when used against the reduced protein identifies bands at 65 Kd. The antibody recognizes only the zymogen of MMP-24, and not the activated forms. The antibody recognizes human and murine MMP-24. The antibody can recognize native or reduced MT5MMP, and has application for immunoprecipitation, immunohistochemistry, cell sorting and ELISA. MT5MMP has been reported to be elevated in several tumor cell lines, and is constituitively produced by some normal cell lines. MMP-24 is also found in brain tissue, along with other MT-MMP enzymes. Treatment of cells with Concanavolin-An or the phorbol ester TPA stimulates production of MT5MMP in some cell types, and the enzyme can be recovered in cell lysates. Shed forms of MT5MMP have also been reported. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentration of antibody may be needed for non-human samples. Researchers should empirically determine the suitability of the Membrane-Type Matrix Metalloproteinase-5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Membrane-Type Matrix Metalloproteinase-5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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