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SDS-Page (Figure 1. SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein, His Tag on SDS-PAGE under reducing condition.)

COVID 19 Nucleocapsid (NP) Coronavirus Recombinant Protein | COVID-19 recombinant protein

SARS-CoV-2 (2019-nCoV) Nucleocapsid, His Tag

Applications
Western Blot, ELISA
Purity
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Synonyms
COVID 19 Nucleocapsid (NP) Coronavirus; SARS-CoV-2 (2019-nCoV) Nucleocapsid; His Tag; 2019 Novel Coronavirus; Coronavirus; CoV; COVID-19 virus; HCoV-2; Human Coronavirus 2019; SARS2; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Nucleocapsid; Nucleoprotein (NP); COVID-19 recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Form/Format
Lyophilized from sterile PBS, pH 7.4. Normally 5%-80% trehalose is added as protectants before lyophilization.
Sequence
Nucleocapsid protein(Met1-Ala419)
Applicable Applications for COVID-19 recombinant protein
Western Blot (WB), ELISA (EIA)
Source
SARS-CoV-2
Conjugation
N-His tag
Reconstitution
Reconstitute with deionized water
Preparation and Storage
Store at -80 degree C for 12 months (Avoid repeated freezing and thawing)
Shipping: Blue Ice

SDS-Page

(Figure 1. SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein, His Tag on SDS-PAGE under reducing condition.)

SDS-Page (Figure 1. SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein, His Tag on SDS-PAGE under reducing condition.)

ELISA (EIA)

(Figure 2. ELISA plate pre-coated by 2 ug/ml (100 ul/well) SARS-CoV-2 (2019-nCoV) Nucleocapsid, His Tag protein can bind Anti-SARS-CoV-2 Nucleocapsid Antibody(DME100015, DME100016) in a linear range of 0.122-15.625 ng/ml.)

ELISA (EIA) (Figure 2. ELISA plate pre-coated by 2 ug/ml (100 ul/well) SARS-CoV-2 (2019-nCoV) Nucleocapsid, His Tag protein can bind Anti-SARS-CoV-2 Nucleocapsid Antibody(DME100015, DME100016) in a linear range of 0.122-15.625 ng/ml.)
Related Product Information for COVID-19 recombinant protein
Coronavirus contain most of nucleocapsid protein. Coronavirus nucleoproteins (N proteins) localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The nucleolus is the site of ribosome biogenesis and sequesters cell cycle regulatory complexes. Two of the major components of the nucleolus are fibrillarin and nucleolin. These proteins are involved in nucleolar assembly and ribosome biogenesis and act as chaperones for the import of proteins into the nucleolus. Regarding of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is a tool for test.

NCBI and Uniprot Product Information

NCBI GeneID
UniProt Accession #
Molecular Weight
The protein has a predicted molecular mass of 49.4 kDa after removal of the signal peptide.

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Product Notes

The COVID-19 (Catalog #AAA188140) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's COVID 19 Nucleocapsid (NP) Coronavirus can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). Researchers should empirically determine the suitability of the COVID-19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Nucleocaps id protein(Me t1-Ala419). It is sometimes possible for the material contained within the vial of "COVID 19 Nucleocapsid (NP) Coronavirus, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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