Rabbit anti-Human Melanocyte Protein PMEL Polyclonal Antibody | anti-PMEL antibody

Melanocyte Protein PMEL, CT (SILV, ME20-M, ME20M, Melanocyte Protein Pmel 17, Melanocytes Lineage-specific Antigen GP100, Melanoma-associated ME20 Antigen, P1, P100, Premelanosome Protein, Silver Locus Protein Homolog, D12S53E, PMEL17)

Cat. Num. AAA6011160

• Gene Names: PMEL; P1; SI; SIL; ME20; P100; SILV; ME20M; gp100; ME20-M; PMEL17; D12S53E
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified; Purified
• Synonyms: Melanocyte Protein PMEL; Polyclonal Antibody; CT (SILV; ME20-M; ME20M; Melanocyte Protein Pmel 17; Melanocytes Lineage-specific Antigen GP100; Melanoma-associated ME20 Antigen; P1; P100; Premelanosome Protein; Silver Locus Protein Homolog; D12S53E; PMEL17); Anti -Melanocyte Protein PMEL; anti-PMEL antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human SILV.
• Purity/Purification: Purified; Purified
• Form/Format: Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 20mg/ml BSA, 0.05% sodium azide.

• Applicable Applications for anti-PMEL antibody: Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Immunohistochemistry.; Dilution: Immunohistochemistry (Formalin-fixed, paraffin-embedded): 5ug/ml
• Immunogen: Synthetic peptide corresponding to the C-terminal sequence of human melanosome/gp100.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human melanoma skin cancer tissue (4 microns) stained with Melanosome antibody shows specific positive immunostaining.)

Immunohistochemistry (IHC)

(Human Prostate (formalin-fixed, paraffin-embedded) stained with SILV antibody at 5ug/ml followed by biotinylated goat rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Related Product Information for anti-PMEL antibody

Melanoma gp100 a melanosomal matrix protein whose expression is closely correlated with cellular melanin content and which is a frequent melanoma tumor antigen recognized by cytotoxic T lymphocytes. It is a likely candidate gene for some cases of human oculocutaneous albinism not associated with known albinism loci. The gp100 protein is the most abundant glycoprotein in nucleated mammalian cells. The gp100 antigen is highly expressed in melanocyte cells, and recognized by cytotoxic T lynphocytes. The gp100 protein is known to initiate cellular immune response through the interaction with mature dendritic cells (DCs). GP100/PMel17 is a melanoma differentiation antigen, which is observed as the target of tumor infiltrating lymphocytes (TIL) and has therefore been proposed as an immunotherapeutic agent in malignant melanoma.

Product Categories/Family for anti-PMEL antibody

Antibodies; Abs to Cancer Markers

NCBI and Uniprot Product Information

• NCBI GI #: 5902084
• NCBI GeneID: 6490
• NCBI Accession #: NP_008859.1
• NCBI GenBank Nucleotide #: NM_006928.4
• UniProt Accession #: P40967; Q12763; Q14448; Q14817; Q16565; B3KS57; B7Z6D7
• Molecular Weight: 70,255 Da
• NCBI Official Full Name: melanocyte protein PMEL isoform 3 preproprotein
• NCBI Official Synonym Full Names: premelanosome protein
• NCBI Official Symbol: PMEL
• NCBI Official Synonym Symbols: P1; SI; SIL; ME20; P100; SILV; ME20M; gp100; ME20-M; PMEL17; D12S53E
• NCBI Protein Information: melanocyte protein PMEL; melanocyte protein mel 17; silver, mouse, homolog of; melanocyte protein Pmel 17; melanosomal matrix protein17; silver locus protein homolog; melanoma-associated ME20 antigen; melanocytes lineage-specific antigen GP100
• UniProt Protein Name: Melanocyte protein PMEL
• Protein Family: Melanocyte protein
• UniProt Gene Name: PMEL
• UniProt Synonym Gene Names: D12S53E; PMEL17; SILV; ME20M; ME20-S; ME20S
• UniProt Entry Name: PMEL_HUMAN

NCBI Description

This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2011]

Uniprot Description

SILV: Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity. Belongs to the PMEL/NMB family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q13-q14

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; plasma membrane; integral to membrane; extracellular region; melanosome

Molecular Function: protein binding

Biological Process: melanin biosynthetic process; melanosome organization and biogenesis