Rabbit PDZK1 Polyclonal Antibody | anti-PDZK1 antibody

PDZK1 (PDZ Domain-containing Protein 1, CFTR-associated Protein of 70kD, CAP70, CLAMP, PDZD1, NHERF3, NHERF-3) (FITC)

Cat. Num. AAA6431097

• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: PDZK1; Polyclonal Antibody; PDZK1 (PDZ Domain-containing Protein 1; CFTR-associated Protein of 70kD; CAP70; CLAMP; PDZD1; NHERF3; NHERF-3) (FITC); anti-PDZK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes PDZK1. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-PDZK1 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:200; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human PDZK1.
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using PDZK1 antibody.)

Product Categories/Family for anti-PDZK1 antibody

Antibodies; PDZ Domain

NCBI and Uniprot Product Information

• NCBI GI #: 55959480
• UniProt Accession #: Q5T2W1; O60450; Q5T5P6; Q9BQ41; B4DPB9; E7EU02
• Molecular Weight: 44,665 Da
• NCBI Official Full Name: PDZ domain containing 1
• UniProt Protein Name: Na(+)/H(+) exchange regulatory cofactor NHE-RF3
• Protein Family: PDZK1-interacting protein
• UniProt Gene Name: PDZK1
• UniProt Synonym Gene Names: CAP70; NHERF3; PDZD1; NHERF-3; NaPi-Cap1
• UniProt Entry Name: NHRF3_HUMAN

Uniprot Description

PDZK1: A scaffold protein that connects plasma membrane proteins and regulatory components, regulating their surface expression in epithelial cells apical domains. May be involved in the coordination of a diverse range of regulatory processes for ion transport and second messenger cascades. In complex with SLC9A3R1, may cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking and the activity of the associated membrane proteins. May play a role in the cellular mechanisms associated with multidrug resistance through its interaction with ABCC2 and PDZK1IP1. May potentiate the CFTR chloride channel activity. May function to connect SCARB1 with the cellular machineries for intracellular cholesterol transport and/or metabolism. May be involved in the regulation of proximal tubular Na(+)-dependent inorganic phosphate cotransport therefore playing an important role in tubule function. Belongs to the NHER family.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: brush border membrane; microvillus membrane; cytoplasm; apical plasma membrane; plasma membrane; lipid raft

Molecular Function: protein binding; scavenger receptor binding; transporter activity; protein complex binding; PDZ domain binding

Biological Process: cell proliferation; drug transport; transport; carnitine transport