Rabbit anti-Human MEKK3 Polyclonal Antibody | anti-MAP3K3 antibody

Anti-MEKK3 antibody

Cat. Num. AAA176176

• Gene Names: MAP3K3; MEKK3; MAPKKK3
• Reactivity: Human
• Applications: Western Blot
• Purity: Immunogen affinity purified.
• Synonyms: MEKK3; Polyclonal Antibody; Anti-MEKK3 antibody; Mitogen-activated protein kinase kinase kinase 3; mitogen-activated protein kinase kinase kinase 3; M3K3_HUMAN antibody; MAP/ERK kinase kinase 3 antibody; MAP3K3 antibody; MAPK/ERK kinase kinase 3 antibody; MAPKKK3 antibody; MEK kinase 3 antibody; MEKK 3 antibody; MEKK3 antibody; mitogen activated protein kinase kinase kinase 3 antibody; Mitogen-activated protein kinase kinase kinase 3 antibody; anti-MAP3K3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 626

• Applicable Applications for anti-MAP3K3 antibody: Western Blot (WB)
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human MEKK3 (11-25aa MNDLVALQMNRRHRM).
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-MEKK3 antibody, MBS176176, Western blottingLane 1: HELA Cell LysateLane 2: A431 Cell LysateLane 3: MCF-7 Cell LysateLane 4: JURKAT Cell Lysate )

Related Product Information for anti-MAP3K3 antibody

Description: Rabbit IgG polyclonal antibody for Mitogen-activated protein kinase kinase kinase 3(MAP3K3) detection. Tested with WB in Human.
Background: MAP3K3(Mitogen-activated protein kinase kinase kinase 3), also known as MAP/ERK KINASE KINASE 3, MEKK3 or MAPKKK3, is an enzyme that in humans is encoded by the MAP3K3 gene. MAP3K3 is contiguously distal to LYK5 on chromosome 17(Puffenberger et al., 2007). By yeast 2-hybrid analysis of a mouse T-cell cDNA library, Uhlik et al.(2003) showed that a C-terminal fragment of mouse Osm(CCM2) interacted with Mekk3, which activates p38 in response to sorbitol-induced hyperosmotic conditions. Mekk3 and Osm colocalized in the cytoplasmic compartment of cotransfected cells, and the Mekk3-Osm complex was recruited to Rac1 and cytoskeletal actin-containing membrane ruffles in response to sorbitol treatment. Protein interaction assays showed that Osm interacted directly with the Mekk3 substrate Mkk3(MAP2K3), with actin, and with both GDP- and GTP-loaded Rac1. Uhlik et al.(2003) concluded that the RAC1-OSM-MEKK3-MKK3 complex is required for regulation of p38 activity in response to osmotic shock.

NCBI and Uniprot Product Information

• NCBI GI #: 160332306
• NCBI GeneID: 4215
• UniProt Accession #: Q99759; Q5BKZ6; Q8N3I9; B2RCW2; D3DU15
• Molecular Weight: 74,089 Da
• NCBI Official Full Name: Mitogen-activated protein kinase kinase kinase 3
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 3
• NCBI Official Symbol: MAP3K3
• NCBI Official Synonym Symbols: MEKK3; MAPKKK3
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 3; MEKK 3; MEK kinase 3; MAP/ERK kinase kinase 3; MAPK/ERK kinase kinase 3
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 3
• UniProt Gene Name: MAP3K3
• UniProt Synonym Gene Names: MAPKKK3; MEKK3; MEK kinase 3; MEKK 3
• UniProt Entry Name: M3K3_HUMAN

NCBI Description

This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

MEKK3: a protein kinase of the STE11 family. Phosphorylated and activated by MLK3 and TAK1. Phosphorylates and activates MEK5.

Protein type: Protein kinase, STE; Kinase, protein; EC 2.7.11.25; Protein kinase, Ser/Thr (non-receptor); STE group; STE11 family

Chromosomal Location of Human Ortholog: 17q23.3

Cellular Component: intracellular; cytosol

Molecular Function: MAP kinase kinase activity; protein binding; MAP kinase kinase kinase activity; metal ion binding; ATP binding; protein kinase activity

Biological Process: blood vessel development; positive regulation of I-kappaB kinase/NF-kappaB cascade; activation of MAPKK activity; activation of MAPK activity; protein amino acid autophosphorylation; MAPKKK cascade