Rabbit MEK3 Polyclonal Antibody | anti-MAP2K3 antibody

Anti-MEK3 antibody

Cat. Num. AAA175436

• Gene Names: MAP2K3; MEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: MEK3; Polyclonal Antibody; Anti-MEK3 antibody; Dual specificity mitogen-activated protein kinase kinase 3(MAP kinase kinase 3/MAPKK 3); mitogen-activated protein kinase kinase 3; AW212142 antibody; Dual specificity mitogen activated protein kinase kinase 3 antibody; Dual specificity mitogen-activated protein kinase kinase 3 antibody; ERK kinase 3 antibody; MAP kinase kinase 3 antibody; MAP2K 3 antibody; map2k3 antibody; MAPK ERK kinase 3 antibody; MAPK kinase 3 antibody; MAPK/ERK kinase 3 antibody; MAPKK 3 antibody; MAPKK3 antibody; MEK 3 antibody; MEK3 antibody; Mitogen activated protein kinase kinase 3 antibody; MKK 3 antibody; MKK3 antibody; mMKK 3b antibody; mMKK3b antibody; MP2K3_HUMAN antibody; MPK 3 antibody; PRKMK 3 antibody; PRKMK3 antibody; Protein kinase mitogen activated kinase 3 antibody; protein kinase; mitogen-activated; kinase 3 antibody; SAPK kinase 2 antibody; SAPKK 2 antibody; SAPKK2 antibody; SKK2 antibody; Stress activated protein kinase kinase 2 antibody; zMKK 3 antibody; anti-MAP2K3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 347

• Applicable Applications for anti-MAP2K3 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human MEK3 (320-334aa MEHPFFTLHKTKKTD), identical to the related mouse and rat sequences.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-MEK3 antibody, MBS175436, Western blottingLane 1: Rat Spleen Tissue LysateLane 2: Rat Thymus Tissue LysateLane 3: Rat Skeletal Muscle Tissue LysateLane 4: Rat Kidney Tissue LysateLane 5: MCF-7 Cell LysateLane 6: HELA Cell LysateLane 7: RAJI Cell LysateLane 8: CEM Cell LysateLane 9: COLO320 Cell Lysate )

Immunohistochemistry (IHC)

(Anti-MEK3 antibody, MBS175436, IHC(P)IHC(P): Human Mammary Cancer Tissue )

Related Product Information for anti-MAP2K3 antibody

Description: Rabbit IgG polyclonal antibody for Dual specificity mitogen-activated protein kinase kinase 3(MAP2K3) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Background: Dual specificity mitogen-activated protein kinase kinase 3 is an enzyme that in humans is encoded by the MAP2K3 gene. The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. Rampoldi et al.(1997) localized the MAP2K3 gene to 17q11.2.

NCBI and Uniprot Product Information

• NCBI GI #: 24638466
• NCBI GeneID: 5606
• UniProt Accession #: P46734; Q99441; Q9UE71; Q9UE72; B3KSK7
• Molecular Weight: 39,940 Da
• NCBI Official Full Name: Dual specificity mitogen-activated protein kinase kinase 3
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 3
• NCBI Official Symbol: MAP2K3
• NCBI Official Synonym Symbols: MEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 3; MEK 3; MAPKK 3; SAPK kinase 2; MAPK/ERK kinase 3; MAP kinase kinase 3; stress-activated protein kinase kinase 2
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 3
• Protein Family: Dual specificity mitogen-activated protein kinase kinase
• UniProt Gene Name: MAP2K3
• UniProt Synonym Gene Names: MEK3; MKK3; PRKMK3; SKK2; MAP kinase kinase 3; MAPKK 3; MEK 3; SAPK kinase 2; SAPKK-2; SAPKK2
• UniProt Entry Name: MP2K3_HUMAN

NCBI Description

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

MKK3: a dual-specificity protein kinase of the STE7 family. Activates p38 MAP kinase by phosphorylating a Thr and a Tyr residue in the activation loop. Is activated by cytokines and environmental stress in vivo. A deletion and two point mutants found in colon cancer cell lines. Three splice-variant isoforms have been described.

Protein type: Protein kinase, STE; Kinase, protein; EC 2.7.12.2; Protein kinase, dual-specificity (non-receptor); STE group; STE7 family

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: nucleoplasm; membrane; cytosol

Molecular Function: MAP kinase kinase activity; protein binding; protein-tyrosine kinase activity; protein kinase binding; ATP binding; receptor signaling protein serine/threonine kinase activity

Biological Process: peptidyl-tyrosine phosphorylation; activation of MAPK activity; MyD88-independent toll-like receptor signaling pathway; positive regulation of transcription, DNA-dependent; stress-activated MAPK cascade; pathogenesis; toll-like receptor 3 signaling pathway; signal transduction; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; positive regulation of protein kinase activity; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; inflammatory response; toll-like receptor 4 signaling pathway; regulation of cytokine biosynthetic process; cardiac muscle contraction