Rabbit MEF2D Polyclonal Antibody | anti-MEF2D antibody

MEF2D (Phospho-S444) Polyclonal Antibody

Cat. Num. AAA3004992

• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: MEF2D; Polyclonal Antibody; MEF2D (Phospho-S444) Polyclonal Antibody; Myocyte-specific enhancer factor 2D; anti-MEF2D antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p-MEF2D (S444) polyclonal antibody detects endogenous levels of MEF2D protein only when phosphorylated at Ser444
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 514

• Applicable Applications for anti-MEF2D antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic phosphopeptide derived from human MEF2D around the phosphorylation site of Serine 444.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Testing Data

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of p-MEF2D (S444) pAb in paraffin-embedded human liver carcinoma tissue at 1:100.)

Related Product Information for anti-MEF2D antibody

The myocyte enhancer factor-2 (MEF-2) family of transcription factors associate with co-repessors or co-activators to regulate development and function of T cells, neuronal cells, and muscle cells. Four family members arise from alternatively spliced transcripts, termed MEF-2A, -2B, -2C, and -2D. These members bind as homo- and heterodimers to the MEF-2 site in the promoter region of affected genes. Differential regulation in the expression of the four transcripts implies functional distinction for each duing embryogenesis and development. The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression. The myogenic basic helix-loop-helix proteins, including MyoD, myogenin, Myf-5, and MRF4, are one class of identified factors. A second family of DNA binding regulatory proteins is the myocyte-specific enhancer factor-2 (MEF-2) family. Each of these proteins binds to the MEF-2 target DNA sequence present in the regulatory regions of many muscle-specific genes.

NCBI and Uniprot Product Information

• NCBI GI #: 410442515
• NCBI GeneID: 4209
• NCBI Accession #: NP_001258558.1
• NCBI GenBank Nucleotide #: NP_001258558.1
• UniProt Accession #: Q14814
• Molecular Weight: ~ 56kDa
• NCBI Official Full Name: myocyte-specific enhancer factor 2D isoform 2
• NCBI Official Synonym Full Names: myocyte enhancer factor 2D
• NCBI Official Symbol: MEF2D
• NCBI Protein Information: myocyte-specific enhancer factor 2D
• UniProt Protein Name: Myocyte-specific enhancer factor 2D
• Protein Family: Myocyte-specific enhancer factor
• UniProt Gene Name: MEF2D
• UniProt Entry Name: MEF2D_HUMAN

NCBI Description

This gene is a member of the myocyte-specific enhancer factor 2 (MEF2) family of transcription factors. Members of this family are involved in control of muscle and neuronal cell differentiation and development, and are regulated by class II histone deacetylases. Fusions of the encoded protein with Deleted in Azoospermia-Associated Protein 1 (DAZAP1) due to a translocation have been found in an acute lymphoblastic leukemia cell line, suggesting a role in leukemogenesis. The encoded protein may also be involved in Parkinson disease and myotonic dystrophy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012]

Uniprot Description

MEF2D: transcription factor which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. May be involved in muscle-specific and/or growth factor-related transcription. Six splice-variant isoforms have been described.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 1q12-q23

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; nucleus

Molecular Function: protein dimerization activity; protein binding; transcription activator binding; histone deacetylase binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; adult heart development; osteoblast differentiation; nervous system development; muscle development; apoptosis; chondrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; endochondral ossification