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Western Blot (WB) (Western blot analysis of extracts of various cell lines, using HNRNPL antibody at 1:1000 dilution.)

Rabbit HNRNPL Polyclonal Antibody | anti-HNRNPL antibody

HNRNPL Polyclonal Antibody

Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Synonyms
HNRNPL; Polyclonal Antibody; HNRNPL Polyclonal Antibody; HNRPL; P/OKcl.14; hnRNP-L; anti-HNRNPL antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH7.4, 150mM NaCl, 0.02% sodium azide and 215% glycerol.
PBS with 0.02% sodium azide, 50% glycerol, pH7.168
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-HNRNPL antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:50-1:200
Immunogen
Recombinant fusion protein of human HNRNPL (NP_001524.2)
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using HNRNPL antibody at 1:1000 dilution.)

Western Blot (WB) (Western blot analysis of extracts of various cell lines, using HNRNPL antibody at 1:1000 dilution.)

Immunofluorescence (IF)

(Immunofluorescence analysis of 293T cells using HNRNPL antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.)

Immunofluorescence (IF) (Immunofluorescence analysis of 293T cells using HNRNPL antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat lung using HNRNPL antibody at dilution of 1:100 .)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded rat lung using HNRNPL antibody at dilution of 1:100 .)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat spleen using HNRNPL antibody at dilution of 1:100 .)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded rat spleen using HNRNPL antibody at dilution of 1:100 .)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human thyroid cancer using HNRNPL antibody at dilution of 1:100 .)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human thyroid cancer using HNRNPL antibody at dilution of 1:100 .)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human esophageal using HNRNPL antibody at dilution of 1:100 .)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human esophageal using HNRNPL antibody at dilution of 1:100 .)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human placenta using HNRNPL antibody at dilution of 1:100 .)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human placenta using HNRNPL antibody at dilution of 1:100 .)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse brain using HNRNPL antibody at dilution of 1:100 .)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse brain using HNRNPL antibody at dilution of 1:100 .)
Related Product Information for anti-HNRNPL antibody
Heterogeneous nuclear RNAs (hnRNAs) which include mRNA precursors and mature mRNAs are associated with specific proteins to form heterogenous ribonucleoprotein (hnRNP) complexes. Heterogeneous nuclear ribonucleoprotein L is among the proteins that are stably associated with hnRNP complexes and along with other hnRNP proteins is likely to play a major role in the formation, packaging, processing, and function of mRNA. Heterogeneous nuclear ribonucleoprotein L is present in the nucleoplasm as part of the HNRP complex. HNRP proteins have also been identified outside of the nucleoplasm. Exchange of hnRNP for mRNA-binding proteins accompanies transport of mRNA from the nucleus to the cytoplasm. Since HNRP proteins have been shown to shuttle between the nucleus and the cytoplasm, it is possible that they also have cytoplasmic functions. Two transcript variants encoding different isoforms have been found for this gene.
Product Categories/Family for anti-HNRNPL antibody

NCBI and Uniprot Product Information

NCBI GeneID
UniProt Accession #
Molecular Weight
MW: 50kDa, 64kDa
SDS PAGE MW: 64kDa
UniProt Protein Name
Heterogeneous nuclear ribonucleoprotein L
UniProt Gene Name
HNRNPL
UniProt Synonym Gene Names
HNRPL; hnRNP L
UniProt Entry Name
HNRPL_HUMAN

Uniprot Description

hnRNP L: This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Is associated with most nascent transcripts including those of the landmark giant loops of amphibian lampbrush chromosomes. Associates, together with APEX1, to the negative calcium responsive element (nCaRE) B2 of the APEX2 promoter. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA splicing; RNA-binding

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: nucleoplasm; membrane; cytoplasm; ribonucleoprotein complex; nucleus

Molecular Function: protein binding; RNA binding; nucleotide binding

Biological Process: nuclear mRNA splicing, via spliceosome; RNA processing; RNA splicing; gene expression

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Product Notes

The HNRNPL hnrnpl (Catalog #AAA9457629) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HNRNPL Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's HNRNPL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC: 1:50-1:200 IF: 1:50-1:200. Researchers should empirically determine the suitability of the HNRNPL hnrnpl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HNRNPL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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