Rabbit anti-Human MED8 Polyclonal Antibody | anti-MED8 antibody

MED8, ID (MED8, Mediator of RNA polymerase II transcription subunit 8, Activator-recruited cofactor 32kD component, Mediator complex subunit 8) (Azide free) (HRP)

Cat. Num. AAA6320071

• Gene Names: MED8; ARC32
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MED8; Polyclonal Antibody; ID (MED8; Mediator of RNA polymerase II transcription subunit 8; Activator-recruited cofactor 32kD component; Mediator complex subunit 8) (Azide free) (HRP); Mediator complex subunit 8; anti-MED8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-MED8 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MED8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-130 amino acids from the Central region of human MED8.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MED8 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the MED8 antibody detected the MED8 protein (arrow).)

Related Product Information for anti-MED8 antibody

This gene encodes a protein that is one of more than 20 subunits of the mediator complex, first identified in S. cerevisiae, that is required for activation of transcription. The product of this gene also interacts with elongins B and C, and CUL2 and RBX1, to reconstitute a ubiquitin ligase. Two alternative transcripts encoding different isoforms have been described.

Product Categories/Family for anti-MED8 antibody

Antibodies; Enzymes; Polymerase

References

Leonard, V.H., et al. J. Virol. 80(19):9667-9675(2006). Wang, A.G., et al. Biochem. Biophys. Res. Commun. 345(3):1022-1032(2006). Sato, S., et al. Mol. Cell 14(5):685-691(2004). Tomomori-Sato, C., et al. J. Biol. Chem. 279(7):5846-5851(2004). Sato, S., et al. J. Biol. Chem. 278(50):49671-49674(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 155030190
• NCBI GeneID: 112950
• NCBI Accession #: NP_443109.2; NP_963836.2
• NCBI GenBank Nucleotide #: NM_052877.4
• UniProt Accession #: Q96G25; Q5JUY8; Q96FQ4; A9IZ91; A9IZ92
• Molecular Weight: 29,080 Da
• NCBI Official Full Name: mediator of RNA polymerase II transcription subunit 8 isoform 3
• NCBI Official Synonym Full Names: mediator complex subunit 8
• NCBI Official Symbol: MED8
• NCBI Official Synonym Symbols: ARC32
• NCBI Protein Information: mediator of RNA polymerase II transcription subunit 8; activator-recruited cofactor 32 kDa component; mediator of RNA polymerase II transcription subunit MED8
• UniProt Protein Name: Mediator of RNA polymerase II transcription subunit 8
• Protein Family: Mediator of RNA polymerase II transcription
• UniProt Gene Name: MED8
• UniProt Synonym Gene Names: ARC32
• UniProt Entry Name: MED8_HUMAN

NCBI Description

This gene encodes a protein component of the mediator complex, which aids in transcriptional activation through interaction with RNA polymerase II and gene-specific transcription factors. The encoded protein may also function in ubiquitin ligation and protein degradation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]

Uniprot Description

MED8: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May play a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins. Belongs to the Mediator complex subunit 8 family. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 1p34.2

Cellular Component: nucleoplasm; Srb-mediator complex

Molecular Function: protein binding

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; protein ubiquitination; gene expression