Typical Testing Data/Standard Curve (for reference only)
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Human Asparagine Synthetase (ASNS) ELISA Kit | ASNS elisa kit
Human Asparagine Synthetase (ASNS) ELISA Kit
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to ASNS. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to ASNS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ASNS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ASNS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]
Uniprot Description
ASNS: 3 isoforms of the human protein are produced by alternative splicing
Protein type: Energy Metabolism - nitrogen; Ligase; EC 6.3.5.4; Amino Acid Metabolism - alanine, aspartate and glutamate; Cell cycle regulation
Chromosomal Location of Human Ortholog: 7q21.3
Cellular Component: cytosol
Molecular Function: protein homodimerization activity; asparagine synthase (glutamine-hydrolyzing) activity; cofactor binding; ATP binding
Biological Process: response to light stimulus; unfolded protein response; response to toxin; glutamine metabolic process; liver development; response to amino acid stimulus; positive regulation of mitotic cell cycle; cellular response to hormone stimulus; cellular response to glucose starvation; cellular protein metabolic process; asparagine biosynthetic process; unfolded protein response, activation of signaling protein activity; response to mechanical stimulus; amino acid biosynthetic process; response to methotrexate; response to follicle-stimulating hormone stimulus; negative regulation of apoptosis
Disease: Asparagine Synthetase Deficiency
Research Articles on ASNS
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Product Notes
The Human ASNS asns (Catalog #AAA2023515) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2023515 ELISA Kit recognizes Human ASNS. It is sometimes possible for the material contained within the vial of "Asparagine Synthetase (ASNS), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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