Rabbit anti-Human Yes1 Polyclonal Antibody | anti-Yes1 antibody

Yes1, NT (Yes1, Yes, Tyrosine-protein kinase Yes, Proto-oncogene c-Yes, p61-Yes) (Azide free) (HRP)

Cat. Num. AAA6364241

• Gene Names: Yes1; Yes; p61-Yes
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Yes1; Polyclonal Antibody; NT (Yes1; Yes; Tyrosine-protein kinase Yes; Proto-oncogene c-Yes; p61-Yes) (Azide free) (HRP); p61-Yes; anti-Yes1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes Yes1. Species crossreactivity: human
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-Yes1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1-30 in the N-terminal region of mouse Yes1, UniProt Accession #NP_033561.1; Q04736. Species sequence homology: rat
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in human placenta tissue lysates (35ug/lane) usingMBS6005813. This demonstrates the Yes1 antibody detected the Yes1 protein (arrow).)

Western Blot (WB)

(Western Blot analysis of Yes1 (arrow) usingMBS6005813. 293 cell lysates (2ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Yes1 gene.)

Product Categories/Family for anti-Yes1 antibody

Antibodies; Protein Kinases

References

Zheng, Y., et al. Mol. Cell. Biol. 30(17):4280-4292(2010). Slanina, H., et al. Infect. Immun. 78(5):1905-1914(2010). Abrami, L., et al. Proc. Natl. Acad. Sci. U.S.A. 107(4):1420-1424(2010). Chen, S.C., et al. Am. J. Physiol., Cell Physiol. 297 (1), C133-C139 (2009): Kao, T.J., et al. J. Neurosci. 29(17):5690-5700(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 6678617
• NCBI GeneID: 22612
• NCBI Accession #: NP_033561.1
• NCBI GenBank Nucleotide #: NM_009535.3
• UniProt Accession #: Q04736
• Molecular Weight: 60,630 Da
• NCBI Official Full Name: tyrosine-protein kinase Yes
• NCBI Official Synonym Full Names: YES proto-oncogene 1, Src family tyrosine kinase
• NCBI Official Symbol: Yes1
• NCBI Official Synonym Symbols: Yes; p61-Yes
• NCBI Protein Information: tyrosine-protein kinase Yes
• UniProt Protein Name: Tyrosine-protein kinase Yes
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: Yes1
• UniProt Synonym Gene Names: Yes

Uniprot Description

Non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Stimulation by receptor tyrosine kinases (RTKs) including EGRF, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly. Participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration. Participates in CD95L/FASLG signaling pathway and mediates AKT-mediated cell migration. Plays a role in cell cycle progression by phosphorylating the cyclin dependent kinase 4/CDK4 thus regulating the G1 phase. Also involved in G2/M progression and cytokinesis ().