Rabbit MED22 Polyclonal Antibody | anti-MED22 antibody

MED22 Polyclonal Antibody

Cat. Num. AAA3006434

• Gene Names: MED22; SRB6; MED24; SURF5; surf-5
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: MED22; Polyclonal Antibody; MED22 Polyclonal Antibody; Mediator of RNA polymerase II transcription subunit 22; Mediator complex subunit 22; Surfeit locus protein 5; Surf-5; SURF5; anti-MED22 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: MED22 polyclonal antibody detects endogenous levels of MED22 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 200

• Applicable Applications for anti-MED22 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human MED22.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of MED22 polyclonal antibody at 1:500 dilutionLane1:A549 whole cell lysateLane2:Mouse heart tissue lysateLane3:Rat liver tissue lysate)

Related Product Information for anti-MED22 antibody

Med22 is a subunit of the RNA polymerase II (Pol II) transcriptional mediator complex. The mediator complex is a coactivator involved in the regulated transcription of Pol II-dependent genes. Functioning as a bridge to convey information from gene-specific regulatory proteins to the basal Pol II transcription machinery, the mediator complex is recruited to promoter regions by directly interacting with regulatory proteins. The mediator complex also serves as a scaffold for the assembly of a functional pre-initiation complex with Pol II and other general transcription factors. Med22 (Mediator complex subunit 22), also known as SURF5 (Surfeit locus protein 5), is a ubiquitously expressed 200 amino acid nuclear protein that is one of several components of the mediator complex. There are two isoforms of Med22 that are produced as a result of alternative splicing events.

NCBI and Uniprot Product Information

• NCBI GI #: 19557695
• NCBI GeneID: 6837
• NCBI Accession #: NP_598395.1
• NCBI GenBank Nucleotide #: NP_598395.1
• UniProt Accession #: Q15528
• Molecular Weight: ~ 22kDa
• NCBI Official Full Name: mediator of RNA polymerase II transcription subunit 22 isoform b
• NCBI Official Synonym Full Names: mediator complex subunit 22
• NCBI Official Symbol: MED22
• NCBI Official Synonym Symbols: SRB6; MED24; SURF5; surf-5
• NCBI Protein Information: mediator of RNA polymerase II transcription subunit 22
• UniProt Protein Name: Mediator of RNA polymerase II transcription subunit 22
• Protein Family: Mediator of RNA polymerase II transcription
• UniProt Gene Name: MED22
• UniProt Synonym Gene Names: SURF5; Surf-5
• UniProt Entry Name: MED22_HUMAN

NCBI Description

This gene encodes a protein component of the mediator complex, which functions in the regulation of transcription by bridging interactions between gene-specific regulatory factors, RNA polymerase II, and general transcription factors. Alternatively spliced transcript variants encoding different isoforms have been observed. [provided by RefSeq, Jul 2013]

Uniprot Description

SURF5: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 22 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 9q34.2

Cellular Component: cytoplasm; Srb-mediator complex

Molecular Function: protein binding

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter